摘要
目的获得低死亡率卡氏肺孢子虫肺炎(Pneumocystis carinii pneumonia,PCP)小鼠模型并缩短诱导时间。方法将雌性C57小鼠随机分为地塞米松实验组、氢化可的松实验组和对照组。地塞米松实验组采用每隔2d皮下注射地塞米松1次的方法诱导PCP;氢化可的松实验组每周皮下注射氢化可的松2次;对照组注射等体积0.9%氯化钠注射液。结果地塞米松实验组至用药第16次(48d)后未见一只小鼠死亡,卡氏肺孢子虫(Pneumocystis carinii,PC)包囊总检出率为86.67%(26/30),与氢化可的松实验组PC包囊总检出率63.33%(19/30)相比,差异具有统计学意义(P<0.05)。地塞米松实验组给药1次(42d)后PC包囊检出率与氢化可的松实验组给药56d后比较,差异无统计学意义(P>0.05)。结论每隔2d皮下注射地塞米松1次可建立低死亡率小鼠PCP模型且缩短诱导时间。
Objective To establish an animal model of pneumocystis carinii pneumonia （PCP） with low mortality. Methods Female C57 mice were divided into dexamethasone,hydrocortisone groups and control group randomly. Mice in dexamethasone group were injected subcutaneously with dexamethasone once every three days. Mice in hydrocortisone group were injected with hydrocortisone twice a week for 9 weeks. Those in the control group were injected with same volume of physiological saline. Results In dexamethasone group, there was no mortality and the detectable rate of PC cysts was 86.7%. In hydrocortisone group, the mortality was 53.33% and the detectable PC cysts rate was 63.33% （ P〈0.05 ）. Conclusion The results showed that immunosuppression with dexamethasone can establish mouse PCP model in shorter time with a low mortality.
出处
《浙江医学》
CAS
2008年第7期704-706,F0004,共4页
Zhejiang Medical Journal
