摘要
本文以壳聚糖代替溶壁微球菌作底物测定溶菌酶的活力。实验证明:在55℃,pH=4.5时,达到最大酶反应速度。酶浓度在0~4.5U/ml范围内符合郎伯—比尔定律,回归方程为Y=0.2173X-0.0114,线性相关系数为γ=0.9998。
Chitosan was used to replace the substrate M.Lysodeikticus to determine the enzyme activity of lysozyme.It showedthat the optimum temperature and optimum pH were 55℃ and 4.5 respectively and it was accordant with the lamber-bier lawwhen the enzyme concentration was 0~4.5U/ml.The regression equation is Y=0.2173X-0.0114 with linear correlationcoefficient as 0.9989.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第9期119-121,共3页
Food Science