摘要
目的探讨一氧化氮合酶 (NOS)抑制剂左旋硝基精氨酸甲酯 (L -NAME)对沙土鼠脑缺血再灌注后海马区胶质纤维酸性蛋白 (GFAP)合成的影响。方法钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用免疫荧光法染色 ,观察GFAP密度的变化及分布。结果脑缺血再灌流后海马区GFAP合成增加 ,GFAP阳性细胞主要分布在放射层及分子层 ,L -NAME能减少海马区GFAP的合成。结论L -NAME是NOS强有力的抑制剂 ,L-NAME可能通过抑制NO的产生抑制了GFAP的合成。
Objective To observe the effects of L-NAME on glial fibrillary acidic protein(GFAP)production in the gerbil hippocampus after recirculation following ischemia.Methods Both vessel occlusion and immunofluorescent methods in gerbil hippocampal tissue slices incubated in vitro were used.Results Re-circulation following ischemia leads to a rise on GFAP concentration in the hippocampus.GFAP positive cells mainly distributed in radial layer and molecular layer of the hippocampus,L-NAME decreased GFAP synthesis.Conclusions L-NAME is a strong inhibitor of nitric oxide synthase(NOS).It is possible that the role of endogenous NO mediated GFAP synthesis is inhibited by L-NAME.
出处
《中国微循环》
2003年第4期232-233,250,共3页
Journal of Chinese Microcirculation
基金
山东省自然科学基金 (NOY2000C23)资助课题
山东省省级中青年学术骨干基金 (369)资助
