摘要
目的:研究糖原磷酸化酶抑制剂-山楂酸对缺血性脑损伤的保护作用,初步探讨糖原磷酸化酶抑制剂的脑缺血保护机制。方法:采用合并迷走神经结扎双侧颈总动脉造成小鼠急性不完全性脑缺血-再灌注模型,测定脑组织匀浆乳酸含量、相关生化指标以及肝糖原含量。结果:山楂酸灌胃给药以后能降低脑组织乳酸、丙二醛含量,提高超氧化物歧化酶、乳酸脱氢酶活力,还能对抗缺血期间的肝糖原降解。结论:山楂酸对缺血性脑损伤具有一定保护作用,其抗脑缺血作用至少是与通过部分抑制糖酵解并进而减轻代谢性酸中毒有关。
AIM： This experiment was designed to study the protective effects of maslinic acid, a novel glycogen phosphorylase inhibitor, on cerebral ischemia in mice. METHODS： The model of acute cerebral ischemia-reperfusion in mice was set up by ligating bilateral common carotid artery and vagus, thus blocking the blood stream for 90 s, and subjecting mice to 5 rain reperfusion. Brain samples were prepared to observe the cerebral injury; liver samples were prepared to observe the status of hepatic glucose metabolism, which contribute to the observed hyperglycemia during cerebral ischemia. RESULTS： Maslinic acid significantly reduced the lactate and MDA level, increased SOD and LDH activity and preserved the hepatic glycogen after mice were subjected to acute cerebral ischemia-reperfusion. CONCLUSION： Maslinic acid may exert its potential protective effects against cerebral ischemic injury, at least in part, through inhibition of over degradation of cerebral glycogen and thus reducing lactate production during ischemia.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第4期381-384,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
教育部"2005年度新世纪优秀人才支持计划"资助项目(NCET-05-0495)
高等学校博士学科点专项科研基金资助项目(Y051018)
关键词
脑缺血
再灌注损伤
乳酸中毒
糖原磷酸化酶抑制剂
山楂酸
cerebral ischemia
ischemia-reperfusion injury
lactate acidosis
glycogen phosphorylase inhibitors
maslinic acid
