摘要
目的:以芡实中总多糖、总酚、总黄酮、不同构型VE含量及抗氧化活性为指标,考察芡实适宜的干燥方式。方法:以紫外分光光度法测定芡实中的总多糖、总酚、总黄酮含量及抗氧化活性,利用高效液相色谱法测定芡实中不同构型VE含量为质量指标,综合评价6种不同干燥方法(阴干、晒干、烘干、真空冷冻干燥、红外干燥、微波干燥)对芡实品质的影响。结果:采用微波干燥能够最大程度地保留样品中总多糖、总酚和总黄酮类物质;在真空冷冻干燥处理条件下,芡实中不同构型VE含量最高且清除1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS+·)的能力最强;相关性分析显示,各不同构型VE含量与抗氧化活性指标具有显著相关性。结论:真空冷冻干燥在最大程度积累VE各类构型成分的基础上,其产品表现出最佳的抗氧化活性,并在一定程度上尽可能地降低了芡实总多糖、总酚及总黄酮类成分的损失,可作为芡实产地加工适宜的干燥方法。
Objective: The contents of total polysaccharides, total phenols, total flavonoids and different configurations of vitamin E and antioxidant activity were considered as indicators to select a suitable drying method for EuryaleSemen. Methods: Ultraviolet(UV) spectroscopy was applied to determine antioxidant activity and the contents of total polysaccharides, total phenols and total flavonoids and different configuration of vitamin E were quantified by high performance liquid chromatography(HPLC). The effects of six different drying methods including shade drying, sun drying, cabinet drying, vacuum-freeze drying, infrared drying and microwave drying on the quality of Euryale Semen were evaluated. Results: Microwave drying could largely retain total polysaccharides, total phenols and total flavonoids in samples. But the highest contents of different configuration of vitamin E were accumulated and the ability to scavenge DPPH free radicals and ABTS+· was the strongest in vacuum freeze-dried samples. Meanwhile, a significant correlation existed between vitamin E and antioxidant activity. Conclusion: Vacuum freeze drying can be regarded as a suitable method for processing Euryale Semen because it not only can largely accumulate the content of vitamin E to result in the best antioxidant activity, but also can reduce the loss of total polysaccharides, total phenols and total flavonoids to the largest extent.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第7期19-25,共7页
Food Science
基金
"十二五"国家科技支撑计划项目(2011BA04B06)
江苏省高校优秀科技创新团队--中药资源化学研究项目(2011)
江苏高校优势学科建设工程资助项目
关键词
干燥方法
芡实
功能性成分
抗氧化活性
drying method
Euryale Semen
functional component
antioxidant activity
