摘要
方格星虫多糖经碱法提取、三氯乙酸脱蛋白、DEAE-52纤维素柱层析、Sephadex G-100凝胶柱层析纯化得到精制方格星虫多糖(Sipunculus nudus polysaccharide,SNPS)。测定SNPS的理化性质和单糖组成,结果表明:SNPS为白色粉末状单一组分;经理化性质测定和紫外光谱分析,表明SNPS的多糖质量分数为99.35%,糖醛酸质量分数为22.21%,不含蛋白质和核酸,不含硫酸根;气相色谱分析结果表明方格星虫多糖SNPS主要由阿拉伯糖、葡萄糖和半乳糖组成,其物质的量比为1.16∶9.54∶1。本研究表明SNPS是从方格星虫中得到的均一组分纯多糖。
Crude polysaccharide was obtained from Sipunculus nudus by alkali extraction and deproteinized by Sevag method. The polysaccharide was sequentially purified by ion exchange chromatography on DEAE-52 cellulose column and Sephadex G-100 Gel column. Then, the purified polysaccharide from Sipunculus nudus(SNPS) was characterized for physicochemical properties and monosaccharide composition. The results showed that SNPS with a purity of 99.35%contained 22.21% alduronic acid without protein, nuclein or sulfate ions. Gas chromatography analysis showed that the monosaccharide compositions of SNPS were arabinose, glucose and galactose, with a molar ratio of 1.16:9.54:1. This study demonstrated that SNPS was a homogeneous component obtained from Sipunculus nudus.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第1期109-112,共4页
Food Science
基金
广西科学研究与技术开发计划项目(桂科攻11107011-6)
广西科学院基本科研业务费资助项目(12YJ25HYS20)
关键词
方格星虫
多糖
纤维素
凝胶
单糖
Sipunculus nudus
polysaccharide
cellulose
gel
monosacharide
