摘要
目的:研究健胃消食片原材料浸膏中多糖的理化性质及其胃排空功能。方法:采用水提醇沉法从健胃消食片浸膏中提取多糖JXP(得率5.44%),通过比色法测定多糖化学成分,高效凝胶渗透色谱检测多糖纯度和相对分子质量,离子交换色谱分析单糖组成,以及利用红外光谱表征多糖红外特征,并进一步通过体内动物实验研究多糖对小鼠胃排空功能的影响。结果:健胃消食片原材料浸膏中多糖的中性糖含量为46.6%,糖醛酸含量为45.7%,蛋白质含量为1.3%。高效凝胶渗透色谱结果显示健胃消食片原材料浸膏中多糖相对分子质量集中在380 000左右。JXP主要由葡萄糖和半乳糖醛酸组成,两者物质的量比为1.2∶1,另含少量阿拉伯糖、半乳糖、甘露糖和木糖。红外光谱图显示JXP含有羧基基团,为吡喃糖构型酸性多糖。胃排空实验发现健胃消食片原材料浸膏中多糖能显著促进小鼠胃排空,特别是当多糖作用剂量为100 mg/kg(以小鼠体质量计)时,效果最为明显。结论:健胃消食片原材料浸膏中多糖为酸性混合杂多糖,其对小鼠胃排空功能具有促进作用。
Objective: To explore the physicochemical properties of the polysaccharide extracted from raw materials used to produce Jianwei Xiaoshi pills(named as JXP), and its effect on gastric emptying activity. Methods: JXP was obtained from Jianwei Xiaoshi pills extract by water extraction and alcohol precipitation with a yield of 5.44%. Chemical composition, purity and relative molecular weight and monosaccharide composition of JXP were determined by colorimetric method, HPGPC and ion exchange chromatography(IEC), respectively. JXP was also characterized by infrared spectroscopy. Then, the effect of JXP on gastric emptying in mice was analyzed. Results: JXP contained 46.6% neutral polysaccharides, 45.7%acidic polysaccharides and 1.3% protein. HPGPC analysis showed that JXP was homogeneous, and its relative molecular weight ranged at around 380 000. Glucose and galacturonic acid were the major monosaccharides in JXP with a molar ratio of 1.2:1. It also contained a small amount of arabinose, galactose, mannose and xylose. Carboxyl groups were contained in the configuration of acidic pyranose. JXP could significantly promote gastric emptying in mice, especially at a dose of 100 mg/kg body weight. Conclusion: JXP contains mixed acidic heteropolysaccharides, which can promote gastric emptying.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第11期37-41,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD33B06)
食品科学与技术国家重点实验室目标导向项目(SKLF-ZZA-201301)
教育部"新世纪优秀人才支持计划"项目(NCET-12-0749)
关键词
健胃消食片
多糖
理化性质
胃排空
Jianwei Xiaoshi pills
polysaccharide
physicochemical properties
gastric emptying
