摘要
mRNA上能发生100多种化学修饰,其中N^6-腺嘌呤(m^6A)是mRNA修饰中最广泛的表观修饰方式之一。在细胞分化、胚胎发育和应激等生物学过程中,特定的mRNA会发生包括N^1-腺嘌呤甲基化、N^5-胞嘧啶甲基化、假尿嘧啶以及N`6-腺嘌呤甲基化等修饰,它们共同形成了mRNA转录后调控的表观修饰转录组,实现对mRNA翻译成蛋白质过程的精确时空调控,特别是m^6A修饰能通过调控mRNA的代谢和翻译等进而调控细胞的一系列生物学过程。文中主要综述mRNA的表观修饰类型和特点,特别是m^6A修饰参与调控mRNA和细胞生物学功能的最新研究进展,并展望了将来m^6A表观修饰的研究重点和方向。
Messenger RNA(mRNA)can be modified by more than 100 chemical modifications.Among these modifications,N^6-methyladenosine(m^6A)is one of the most prevalent modifications.During the processes of cells differentiation,embryo development or stress,m^6A can be modified on key mRNAs and regulate the progress of cells through modulating mRNA metabolism and translation.Other mRNA modifications,including N^1-methyladenosine(m^1 A),5-methylcytosine(m^5 C)and pseudouridine,together with m^6A form the epitranscriptome of mRNA that accurately modulate the mRNA translation.Here we review the types and characteristic of mRNA epigenetic modifications,especially the recent progresses of the function of m^6A,we also expect the main research direction of m^6A epigenetic modification in the future.
作者
甘海丽
洪岭
杨凤莲
柳定凤
金莉萍
郑青亮
Haili Gan;Ling Hong;Fenglian Yang;Dingfeng Liu;Liping Jin;Qingliang Zheng(Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital,Tongji University School of Medicine,Shanghai 201204,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2019年第5期775-783,共9页
Chinese Journal of Biotechnology
基金
国家自然科学基金(Nos.81730039
81671460
81871167)
上海市自然科学基金(No.18ZR1430000)资助~~
关键词
mRNA表观修饰
m^6A
m^1A
假尿嘧啶
mRNA代谢
细胞分化
epigenetic modification of mRNA
N^6-methyladenosine(m^6A)
N^1-methyladenosine(m^1A)
pseudouridine
mRNA metabolism
cell differentiation
