摘要
目的 :探讨湖北地区汉族健康人群白细胞介素 1受体拮抗剂(IL 1Ra)内含子 2基因多态性的分布 ,比较其在不同种族间分布的差异。方法 :采用PCR方法检测了 2 5 1例湖北地区汉族健康人群IL 1Ra基因内含子 2的可变数串联重复 (VNTR)多态性 ,并结合文献进行不同种族间的比较分析。结果 :湖北地区汉族健康人群基因型以Ⅰ /Ⅰ型最为常见 ,其次为Ⅰ /Ⅱ型 ,Ⅰ /Ⅳ和Ⅱ /Ⅱ型较为罕见 ,分布频率依次为 0 .813、0 .167、0 .0 16、0 .0 0 4;其等位基因以Ⅰ型最为常见 ,其次为Ⅱ型 ,Ⅳ型较为罕见。与美、德和日本等国家人群相比 ,该VNTR多态性均存在显著性差异 (P <0 .0 0 5 ) ,与国内江苏和重庆地区人群相比虽无显著性差异 (P >0 .0 5 ) ,但在湖北地区发现了较为罕见的Ⅰ /Ⅳ和Ⅱ /Ⅱ基因型。结论 :湖北地区汉族人群IL 1Ra基因内含子 2存在VNTR多态性 。
Objective: To observe the variable number of tandem repeat (VNTR polymorphism in the IL 1RN (intron 2) in the healthy Chinese Han Population of Hubei, and analyze the distributions of VNTR polymorphism among different races.Method: Using polymerase chain reaction method,To detected the VNTR polymorphism in 251 healthy people.Results: In Han population of Hubei, the Ⅰ/Ⅰgenotype was the most common, then the Ⅰ/Ⅱ genotype, but the Ⅰ/Ⅳ and Ⅱ/Ⅱ genotypes were rare, whose frequency were 0.813,0.167,0.016 and 0.004 respectively. Compared with American, Germany and Japanese populations, the VNTR polymorphism were significantly different( P <0.05), but similar with Jiangsu and Chongqing populations in China( P >0.05).However, we found the rare Ⅰ/Ⅳ and Ⅱ/Ⅱ genotypes Compared with other populations in China.Conclusion: There is a VNTR polymorphism in the IL 1RN (intron 2) in Chinese Han population of Hubei, whose distribution are significantly different in different ethnic populations.
出处
《微循环学杂志》
2003年第3期27-29,共3页
Chinese Journal of Microcirculation
