摘要
目的 探讨湖北地区汉族健康人群脂多糖受体(CD14)启动子T 260C位点多态性的分布。方法 采用聚合酶 链反应和限制性片段长度多态性方法检测152名湖北地区汉族健康人群CD14启动子( 260)位点多态性,并结合文献进行不 同种族间的比较分析。结果 湖北地区汉族健康人群基因型频率依次为TC44.7%、TT28.3%、CC27.0%;其等位基因T和 C的频率分布相近。与捷克、德国、日本、美国人群相比,该多态性位点分布与捷克人群间存在极显著性差异(P<0.01)。结 论 湖北地区汉族人群CD14启动子 260位点存在T/C多态性,其在不同种族间的分布可能存在显著性差异。
Objective To observe the single nucleotide polymorphism (SNP) in the promoter of CD14 at position -260 in the health Chinese Han population of Hubei.Methods Using PCR-RFLP method, the SNP in 152 healthy people was detected, and the genotype and allele frequencies were analyzed among different races according to references.Results In Han population of Hubei, the frequency of each genotype was TC 44.7 % , TT 28.3 % and CC 27.0 % respectively. Compared with Czech, German, Japanese and American populations, the distributions of the SNP were significantly different (P< 0.01 ).Conclusion There is a SNP at position -260 in the promoter of CD14 in Chinese Han population of Hubei, whose distributions are probably significantly different in different ethnic populations.
出处
《华中医学杂志》
2005年第1期16-17,F004,共3页
Central China Medical Journal
