摘要
目的 :采用高效液相色谱 (HPLC)法同时测定滴鼻剂中盐酸麻黄碱、盐酸苯海拉明、地塞米松磷酸钠的含量。方法 :采用C18色谱柱 ,流动相为甲醇 0 .0 5mol·L- 1KH2 PO4 -三乙胺 (58∶42∶0 .2 ) ,检测波长为 2 2 1nm。结果 :盐酸麻黄碱在 160 0～ 2 40 0mg·L- 1浓度范围内线性关系良好 (r =0 .9994) ,回收率为 99.8% ,RSD为 1.0 %。盐酸苯海拉明 80～ 12 0mg·L- 1浓度范围内线性关系良好 (r =0 .9996) ,回收率为 10 1.0 % ,RSD为 0 .9%。地塞米松磷酸钠 160～ 2 40mg·L- 1浓度范围内线性关系良好 (r =0 .9991) ,回收率为 99.6% ,RSD为 0 .8%。结论 :该方法可同时测定滴鼻剂中盐酸麻黄碱、盐酸苯海拉明。
OBJECTIVE To establish a method for simultaneous determination of ephedrine hydrochloride , diphenhydramine and dexamethasone sodium phosphate in nose drops by HPLC. METHODS Ephedrine hydrochloride,diphenhydramine and dexamethasone sodium phosphate were eluted with CHOH 3- 0.05 mol·L -1 KH 2PO 4(CH 3CH 2)3N(58∶42∶ 0 .2 ) on C 18 column and detedted at 221 nm. The pH of the mobile phase was adjusted to 3.0 with phosphoric acid. RESULTS The linear range was 1600～2400 mg·L -1 (r=0.9994), the average recover y was 99.8% , RSD= 1.0% for ephedrine hydrochloride, The linear range was 80～120 mg·L -1 (r=0.9996),the average recovery was 101.3% ,RSD= 0. 9% for diphenhydramine, The linear range was 160～240 mg·L -1 (r= 0. 9991 ),the average recovery was 99.6% ,RSD= 0.8% for dexamethasone sodium phosphate.CONCLUSIONS This method was accurate for simultaneous determination of the three components in nose drops.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2003年第8期480-481,共2页
Chinese Journal of Hospital Pharmacy
