摘要
为了探讨氯化甲基汞(MMC)对大鼠海马损伤的分子机制,应用免疫组织化学方法观察了氯化甲基汞对大鼠海马JUN蛋白表达的影响(对照组为0.9%生理盐水、暴露组浓度分别为0.05,0.5,5mg/kg;取样时间分别为20,60,240,1440min).结果发现,暴露组大鼠海马JUN蛋白表达均高于对照组,氯化甲基汞对大鼠海马JUN蛋白表达与其浓度之间有一定的剂量反应关系,表明即刻早期基因(c-jun)参与了氯化甲基汞对海马损害的毒性过程.
In order to probe into the molecular mechanism of injury in rat hippocampus by methylmercuric chloride (MMC), the effect of MMC on the expression of JUN protein in rat hippocampus was observed using immunocyto-chemical method (the control group was physiological saline of 0.9%, the concentrations of expose groups were 0.05, 0.5, 5mg/kg respectively, the sampling times were 20, 60, 240, 1440min). The expression of JUN protein in rat hippocampus of the expose groups was higher than that of the control group; there was a definite dose-response relationship between concentration of methylmercury chloride and its expression of JUN protein, indicating that IEG (c-jun) participated in the toxicity process of hippocampus injury by methylmercuric chlorid.
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2003年第4期431-434,共4页
China Environmental Science
基金
国家自然科学基金资助项目(20177013)
上海交通大学985工程资助项目(113516001)
关键词
氯化甲基汞
海马
C-JUN
基因表达
methylmercuric chloride
hippocampus
c-jun
gene expression
