摘要
目的 建立人胚骨髓间充质干细胞 (MSCs)分离及培养的方法 ,探讨体外培养MSCs的生物学特性 .方法 通过密度梯度离心、贴壁法分离培养人胚MSCs,在倒置显微镜下连续观察细胞的形态变化 .应用流式细胞术测定细胞周期和CD44、CD3 4 、CD45表达 ,并研究其增殖及生长特征 .结果 原代及传代培养显示 ,14代以前的MSCs具有活跃的增殖能力 ,细胞周期分析显示有 82 %的MSCs处于G0 /G1期 .MSCs阳性表达CD44,阴性表达CD3 4 、CD45.结论 体外培养 14代以前的的MSCs生长稳定 ,增殖较快 。
Objective To establish a method of isolation and cultivation of human bone marrow mesenchymal stem cells(MSCs)in vitro and investigate the biological characteristics of MSCs. Methods Human fetal MSCs were isolated and cultured in DMEM. The morphology of MSCs was observed with inserted microscope constantly. Cell cycle and surface marker were analyzed by flow cytometry. The growth characteristic of MSCs was measured during primary and passage culture. Results MSCs showed active proliferative ability in primary and passage cultere before the 14 th . Cell cycle analysis showed that 82% of MSCs was in G 0/G 1 phase. The cells were positive for CD 44 and negative for CD 34 ,CD 45 . Conclusion Before the 14 th passages,MSCs showed stable and rapid growth in vitro, MSCs could be used as the seed cells for tisuue engineering.
出处
《现代临床医学生物工程学杂志》
2003年第3期177-179,共3页
Journal of Modern Clinical Medical Bioengineering
基金
广州医学院课题
