摘要
目的:利用膜引导组织再生技术,将复合异体骨髓基质细胞(BMSCs)的明胶海棉移植于硅胶膜管内,旨在观察异体BMSCs促进膜引导骨组织再生的能力。方法:抽取大耳白兔胫骨结节及股骨转子处骨髓,经密度梯度离心,贴壁筛选,获取BMSCs并培养、扩增。36只成年新西兰兔随机分成3组,每组12只,造成双侧12mm桡骨缺损,A组缺损区移植吸附异体BMSCs的明胶海绵,B组缺损区移植吸附自体BMSCs的明胶海绵。C组缺损区移植吸附生理盐水的明胶海绵。分别于术后第2,4,8,10周摄X线片,动态观察新生骨痂生长及骨组织愈合情况;于术后第2,4,8,10周取材进行组织学染色,观察骨缺损修复过程中的组织形态学变化;于术后第8,10周行抗弯曲负荷实验。结果:A组与B组移植区骨再生活跃,新骨增生明显,10周骨缺损完全修复,C组各时间点骨修复能力均较A、B组差。组织形态学显示A组与B组新骨形成方式及骨愈合时间两者无明显差异;但两者明显优于C组;术后第8,10周抗弯曲负荷实验表明A组与B组生物力学指标两者差异无显著性(P>0.05),但均高于C组(P<0.05)。结论:异体BMSCs联合引导性骨再生技术可顺利完成骨缺损的修复,其可以成为生物工程种子细胞的理想选择。
Objective：To investigate whether allogeneic bone marrow stromal cells would facilitate bone healing combined with guided bone regeneration（GBR）.Methods：Bone marrow stromal cells were obtained from tibial tuberosity and femur greater trochanter of New Zealand rabbits.A 12 mm long segmental defect was created in the diaphyses of both radii of rabbits.The defect was bridged with a silicon tube.The group A were instilled with allogeneic bone marrow stromal cells into the tube,group B were instilled with autologous bone marrow stromal cells,while group C were only treated with 0.9% normal saline.The specimens were collected at the end of the end of 2nd,4th,8th,10th weeks postoperatively.X-ray,histological examination,HE staining,biochemical examinations and biomechanical examination were applied to observe the repairing of bone defects and guided bone regeneration.Results：The new bone formation of group A was not different from that of group B（P0.05）,but the new bone formation of group A and B was more than that of group C.All the defects of group A and B had been healed at the end of the 10th week,but no one healed in group C.The strength of the newly formed bone had no difference between group A and group B in 8 and 10 weeks postoperatively（P0.05）,but it was higher both in group A and group B than in group C（P0.05）.Conclusion：Allogeneic bone marrow stromal cells can stimulate bone formation and facilitate GBR in bone defect.Allogeneic bone marrow stromal cells can provide ideal source of seed cells for bone tissue engineering.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2011年第5期600-603,I0002,共5页
Medical Journal of Wuhan University
关键词
骨髓基质细胞
移植
引导性骨再生
骨缺损
修复
Bone Marrow Stromal Cells
Transplantation
Guided Bone Regeneration
Bone Defect Reparation
