摘要
目的 观察流感病毒对肿瘤细胞是否具有凋亡诱导作用 ,并进一步研究 Fas L 在此过程中的作用。方法 流感病毒以 2 0 m.o.i.感染 Hela、Raji、SMMC- 772 1和 SPC- A- 1等肿瘤细胞 ,于诱导后不同时间观察细胞超微结构改变 ,DNA琼脂糖凝胶电泳检测梯状条带 ,PI染色流式细胞仪检测细胞 DNA含量 ,Annexin- V FITC/ PI流式细胞仪进行凋亡定量分析 ,并用生物素 -亲和素 EL ISA测定流感病毒诱导后细胞培养上清中 s Fas L 浓度的变化。结果 经流感病毒诱导后 ,四种肿瘤细胞均显示凋亡的形态学改变 ,细胞 DNA电泳出现梯状条带 ,流式细胞仪测定表明细胞凋亡率增高 ,且显示一定的时间效应 ;在上述过程中 ,细胞培养上清中 s Fas L 浓度有明显增高。结论 流感病毒可诱导上述肿瘤细胞发生凋亡 ,其发生机制可能与 Fas L
Objective To study the apotosis-inducing effects of influenza virus on cancer cells and the role of FasL. Methods Four strains of cancer cells (Hela, Raji, SMMC-7721 and SPC-A-1) were infected with 20 m.o.i influenza virus and the effects of apoptosis were detected at different time points post infection by electron microscopy, DNA agrose gel electrophoresis, PI stained flow cytometry, and Annexin-V FITC/PI stained flow cytometry. And the concentration of FasL in the culture supernatant was measured with the use of ELISA at the same time. Results All the four strains of cancer cells showed apoptotic morphological changes. DNA agarose electrophoresis also showed 'ladder' in a time dependent manner after virus infection. During this course, there was an obvious increase of FasL concentration in the cell culture supernatant. Conclusion Influenza virus can induce tumour cells apoptosis in vitro, and it is probably related to the increased expression of FasL.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期409-412,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金 (批准号 39970 830 )资助
