摘要
目的:通过检测不同时相食积复合流感病毒(FM1)感染小鼠血清免疫球蛋白M(IgM)、IFN-γ、IL-4及TH1/TH2水平,探讨食积对流感病毒感染(FM1)小鼠免疫功能的影响。方法:通过喂饲小鼠高蛋白、高热量特制饲料复制食积模型,在此基础上,经鼻感染FM1甲型流感病毒,复制病毒性感染模型,于感染后第1、3、5天,分别检测各组小鼠血清IgM、IFN-γ、IL-4及TH1/TH2水平。结果:小鼠感染FM1流感病毒后第5天,单纯感染组、食积感染组小鼠血清IgM含量均较正常组下降,且食积感染组结果差异有统计学意义(P<0.05),食积感染组较单纯感染组正常组血清IgM含量明显降低,且结果有统计学意义(P<0.01);病毒感染后第1、3、5天,单纯感染组、食积感染组小鼠血清TH1(IFN-γ)/TH2(IL-4))水平均较正常组降低,且在第3天差异有统计学意义(P<0.05),第3、5天食积感染组较正常组降低更为明显,且差异有统计学意义(P<0.05)。结论:食积可能通过影响机体IgM、IFN-γ、IL-4及TH1/TH2水平对流感病毒感染(FM1)小鼠的免疫失衡产生影响,从而提示食积因素可能对流感的发生发展产生了影响。
Objective： To investigate the impact of dyspepsia on immune function on influenza virus（FM1） infected mice,through detecting the serum levels of immunoglobulin M（IgM）,Interferon-γ（IFN-γ）,Interleukin-4（IL-4） and TH1 / TH2.Methods： The mice dyspepsia model was built by feeding mice with high protein-calorie food,then nasal infected with FM1 influenza A virus,randomized to normal group,infected group,and infected and dyspepsia group on D3 and D5 after infection,detected the serum level of IgM,IFN-γ,IL-4 and TH1 / TH2 levels.Results： Mice were infected with FM1 virus at D5,the IgM level of both infected groups were significantly higher than that of the normal group（P 0.05）.The dyspepsia group showed significantly decreased IgM level（P 0.01） compared with the virus infected group;at D1,D3,D5,the serum level of TH1（IFN-γ） / TH2（IL-4） of the infected groups were lower than that of the normal group,and the reduction was siginificant on D3（P 0.05）;at D3 and D5 the dyspepsia group was even more lower（P 0.05）.Conclusion： Dyspepsia can break the balance of immune system by impacting IgM,IFN-γ,IL-4 and TH1/TH2,indicating dyspepsia plays a role in influenza epidemic.
出处
《世界中医药》
CAS
2013年第9期1085-1087,1090,共4页
World Chinese Medicine
基金
科学研究与研究生培养共建项目--科研项目--应用队列研究序贯扎根理论探索中医病因学研究的新模式(编号:521/0101204)
北京市教委共建项目资助(编号:0101165)
北京中医药大学自主课题(编号:2009JYBZZ-JS008)
北京中医药大学青年教师专项课题:食积与肺肠损伤的相关性实验研究
关键词
食积
流感病毒
免疫功能
Dyspepsia
Influenza Virus
Immune function
