摘要
目的 :分析结肠癌相关转录因子1(CCAT-1)m RNA在结直肠癌患者外周血中的表达水平与临床病理特征及预后生存的关联,探讨CCAT-1在结直肠癌早期筛查及病程预后评估中的作用。方法:采用实时定量PCR法(q PCR)检测结直肠癌(60例)、结直肠腺瘤(30例)及健康志愿者(30例)外周血中CCAT-1 m RNA表达,采用ROC曲线判断CCAT-1对结直肠癌的诊断价值,计算Youden指数确定CCAT-1对结直肠癌的诊断阈值;采用双变量相关(bivariate correlation)Pearson检验分析CCAT-1m RNA表达水平与结直肠癌患者临床病理特征;采用Kaplan-Meier生存分析(Log-rank检验)分析CCAT-1 m RNA表达与结直肠癌患者3年生存率的相关性,绘制生存曲线。结果:结直肠癌患者外周血CCAT-1 m RNA水平相对于结直肠腺瘤患者(P<0.01)、健康志愿者(P<0.01)显著高表达,CCAT-1 m RNA在健康志愿者、结直肠腺瘤及结直肠癌患者外周血中的表达量呈逐渐递增趋势;结直肠癌患者外周血CCAT-1 m RNA表达量与肿瘤直径(P<0.05)、分化程度(P<0.01)、原发灶浸润深度(P<0.01)、淋巴结转移数(P<0.01)、远处转移(P<0.05)及TNM分期(P<0.05)呈显著正相关,与3年生存率呈负相关(P<0.05);CCAT-1 m RNA高表达组生存时间显著低于低表达组(P<0.01)。结论:CCAT-1可作为一种有价值的结直肠癌早期筛查及预后评估的标志物。
Objective:To investigate the relation between m RNA expression of CCAT-1 in the peripheral blood, clinicopathological features and prognosis of patients with colorectal carcinoma. Methods: Real-time quantitative polymerase chain reaction(q PCR) was performed to detect CCAT-1 m RNA expression levels in patients with colorectal carcinoma(60 cases) and colorectal adenoma(30 cases)as well as healthy volunteers(30 cases). The value of CCAT-1 in diagnosis of colorectal carcinoma was evaluated by ROC curve, diagnosis threshold of CCAT-1 m RNA level in colorectal carcinoma was determined by Youden index. Bivariate correlation analysis was performed to analyze the correlation of the expression levels of CCAT-1 and clinicopathological features in patients with colorectal carcinoma. Kaplan-Meier survival curve was performed to analyze the correlation between CCAT-1 expression and survival of patients with colorectal carcinoma. Results: CCAT-1 m RNA expression levels in patients with colorectal carcinoma were significantly higher than those in patients with adenoma(P < 0.01) and healthy volunteers(P < 0.01). CCAT-1 m RNA expression levels were positively correlated with the diameters of tumor, grade of differentiation, invasion depth of primary tumor, the number of lymph node anddistant metastasis(P < 0.05) and TNM stage(P < 0.01), and were negatively correlated with survival time(P < 0.05). The survival time of patients with colorectal carcinoma with higher-level CCAT-1 expression was significantly shorter than that of the lower-level group. Conclusion: CCAT-1 can be considered as a valuable marker for early screening and prognosis assessment of colorectal carcinoma.
出处
《南京医科大学学报（自然科学版）》
CSCD
北大核心
2015年第7期1008-1012,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
湖州市科技公益性技术应用研究(2013GY19)
湖州市自然科学基金(2014YZ04)
浙江省医药卫生研究计划(2015KYB372)
