摘要
目的观察黄芩汤对结肠炎相关结肠癌小鼠模型Notch/Wnt信号通路的影响。方法 C57BL/6雄性小鼠随机分为正常组、模型组、低剂量和高剂量黄芩汤组,用氧化偶氮甲烷联合葡聚糖硫酸钠建立结肠炎癌变动物模型。观察疾病活动指数(DAI)、结肠长度和肠道肿瘤数量,ELISA检测血清促炎因子TNF-α、IL-6和IL-1β含量,免疫组织化学检测结肠上皮细胞增殖、凋亡及Notch/Wnt信号通路中关键蛋白表达。结果黄芩汤显著降低模型小鼠DAI评分和肿瘤数量,增加结肠长度,抑制结肠上皮细胞增殖并促进凋亡,下调促炎因子(TNF-α、IL-6、IL-1β)、Notch-1、Hes-1、β-catenin、c-myc表达。结论黄芩汤可有效预防结肠炎相关结肠癌的发生与发展,其机制可能涉及Notch/Wnt信号通路的抑制。
Objective To investigate the effect of Huangqin Tang（HQT） on Notch/Wnt pathway in mice with colitisassociated colon cancer（CACC）. C57 BL/6 male mice were randomized to normal, model, and low-and high-dose MethodsHQT groups. CACC models were established using azoxymethane and dextran sulfate sodium. The disease activity index（DAI）, colon length and number of tumors were observed. Serum levels of proinflammatory cytokines（TNF-α, IL-6 and IL-1β）, proliferation, apoptosis, and key protein expression of Notch/Wnt pathway were determined by ELISA and immunohistochemistry. Results HQT significantly decreased DAI and tumor number but increased colon length. Furthermore, HQT dramatically suppressed proliferation but enhanced apoptosis, and down-regulated expression of proinflammatory cytokines（TNF-α, IL-6 and IL-1β）, Notch-1, Hes-1, β-catenin, and c-myc. Conclusion HQT may prevent the development and progression of CACC via inhibiting Notch/Wnt signaling.
作者
迟宏罡
张淑华
于丰彦
江粤媚
卢彭信
官学苹
阮奇军
周桂海
邹颖
CHI Hong-gang;ZHANG Shu-hua;YU Feng-yan;JIANG Yue-mei;LU Peng-xin;GUAN Xue-ping;RUAN Qi-jun;ZHOU Gui-hai;ZOU Ying(Department of Traditional Chinese Medicine,Second Clinical Medical College;Department of Internal Medicine,Second Clinical Medical College;Grade 2015 Traditional Chinese Medicine Specialty,College of Pharmacy;Grade 2016 Stomatology Specialty,Second Clinical Medical College;Sino-American Cancer Research Institute,Dongguan Research Center;Guangdong Medical University,Dongguan 523808,China)
出处
《广东医科大学学报》
2018年第4期375-380,共6页
Journal of Guangdong Medical University
基金
国家自然科学基金资助项目(No.81541166
No.81573932
No.81603592)
东莞市社会科技发展项目(No.2014108101054)
广东省自然科学基金项目(No.2016A030310356)
