摘要
目的:探讨结直肠癌患者血清血管内皮生长因子(VEGF)和基质金属蛋白酶-2(MMP-2)水平的变化和意义。方法:采用双抗体夹心法检测72例结直肠癌患者(术前、术后3个月、术后1年)、30例结直肠腺瘤患者和30例健康体检者血清VEGF和MMP-2的水平。结果:结直肠癌组患者术前血清VEGF和MMP-2水平明显高于结直肠腺瘤组和正常对照组(P<0.05);术后3个月,72例结直肠癌患者血清VEGF和MMP-2水平明显低于术前(P<0.05),与正常对照组比较差异无统计学意义(P>0.05)。结直肠癌患者血清VEGF和MMP-2水平与年龄、性别、组织类型、大体分型、肿瘤部位无关(P>0.05);与Dukes分期、分化程度、淋巴结转移、浸润深度、肿瘤大小有关(P<0.05)。术后随访1年时,复发者血清VEGF和MMP-2水平明显高于未复发者(P<0.05)。结论:血清VEGF和MMP-2水平可能在结直肠癌的早期诊断、病情评估及预后评价中具有重要作用。
Objective：To investigate the changes and significance of the vascular endothelial growth factor（VEGF）and matrix metalloproteinase-2（MMP-2） level in serum of colorectal cancer patients.Methods：Double antibody sandwich method was used to detect the serum VEGF and MMP-2 level of 72 colorectal cancer patients（before operation,3 months and1 year after operation）,30 colorectal adenomas patients and healthy controls.Results：The serum VEGF and MMP-2 level of colorectal cancer patients before operation were obviously higher than that of colorectal adenomas patients and healthy controls（P〈0.05）;3 months after operation,the serum VEGF and MMP-2 level of colorectal cancer patients were significantly lower than that before operation（P〈0.05）,and had no statistical significance compared with healthy controls（P〈0.05）.The serum VEGF and MMP-2 level of colorectal cancer patients were not related to age,sex,histological type,gross classification and tumour location（P〈0.05）;but related to Dukes stage,differentiated degree,lymph node metastasis,depth of infiltration and tumour size（P〈0.05）.1 year after operation,the serum VEGF and MMP-2 level of relapsed patients were obviously higher than that of no relapsed patients（P〈0.05）.Conclusions：The serum VEGF and MMP-2 level may play important role in early diagnosis,severity assessment and prognostic evaluation of colorectal cancer.
出处
《承德医学院学报》
2016年第1期3-5,共3页
Journal of Chengde Medical University
