摘要
采用反相高效液相色谱技术 ,分离制备粗儿茶素粉末中表没食子儿茶素没食子酸酯( EGCG)、没食子儿茶素没食子酸酯 ( GCG)、表儿茶素没食子酸酯 ( ECG)三种酯型儿茶素馏分。制备色谱条件 :色谱柱 ,( 80 mm i.d× 5 0 0 mm,Fuji C18,粒径 2 5～ 40 μm) ;流动相 :有机醇 A- 1 /水( 1 6.5 /83.5 ,v/v,流速为 5 8m L/min;检测波长 2 78nm,进样量 30 0 0 mg。将所收集相应馏分分别经 P- 1大孔树脂吸附脱去溶剂 ,低沸点溶剂洗脱 ,旋转蒸发浓缩洗脱液 ,冷冻干燥后 ,得到 EGCG、GCG、ECG白色粉末。各物质经 HPLC检测纯度达 99.2 %以上。该法适用于分离纯化 EGCG、GCG。
Separation and preparation of (-) epigallocatechin(EGCG), (-) gallocatechin gallate(GCG) and (-) epicatechin gallate(ECG) from raw power of catechins were investigated by HPLC. Preparative chromatography conditions were: column(80 mm i.d×500 mm, Fuji C 18 , grain size 25~40 μm); mobile phase(alcohol A 1/water, 16.5/83.5, v/v ), flow rate(58 mL/min); detective wave length(278 nm); injection volume(10 mL). Under these conditions, three kinds of ester catechins were collected separately. Adsporptive resin P 1 was used to get rid of the solvent and low boiling point solvent was used to elute the above components. The eluted solution was evaporated and freeze dried under vacuum. Three kinds of ester catechin monomers can be obtained and their purity is more than 99.2%. The method is appicable for industrial production of ester catechin monomers-EGCG、GCG、ECG.
出处
《化学世界》
CAS
CSCD
北大核心
2003年第5期237-239,245,249,共5页
Chemical World
基金
湖南省自然科学基金项目 (0 IJJY2 0 98)
湖南省重点科技攻关项目 (0 0 NKY10 10 )
关键词
高效制备液相色谱
分离和制备
酯型儿茶素
high performance liquid chromatography
separation and preparation
ester catechin
