摘要
采用大鼠离体工作的心脏 ,观察不同浓度的中药复方益心汤对大鼠心肌缺血再灌注损伤的保护作用。将 30只Wistar大鼠随机分为 3组 ,益心汤 1组 (YXT1,每ml平衡液中含 0 0 1g中药生药 ) ,益心汤 2组(YXT2 ,每ml平衡液中含 0 1g中药生药 )和对照组 ;以左室内压峰值 (LVSP) ,左室内压最大上升和下降速率 (±dp/dtmax) ,左室舒张末期的压力 (LVEDP) ,心率(HR) ,心肌组织的丙二醛 (MDA)含量的变化作为观察指标。结果显示 ,益心汤可明显改善心肌缺血再灌注后的心肌力学 (±dp/dtmax)和血液动力学 (LVSP)等指标 ,并可降低心肌组织的MDA含量 ,减少氧自由基的产生 ,减轻再灌注的损伤 ,益心汤 2组明显优于对照组(P <0 0 5 )。提示中药复方益心汤具有保护心肌缺血再灌注损伤的作用 。
To observe the effect of 'Yixintang' on the protection of the myocardium against ischemia reperfusion injury. Thirty Wistar rats, with their hearts isolated, were randomly divided into three groups: Yixintang group 1 (YXT1, with 0 01g 'Yixintang' in each ml K H fluid), Yixintang group 2 (YXT2, with 0 1g 'Yixintang' in each ml K H fluid) and control group. Left ventricular systolic output pressure (LVSP), rate of rise of left ventricular pressure (±dp/dt max ), left ventricular end diastolic pressure (LVEDP), heart rate, the content of malondialdehyde(MDA) in myocardial tissue were measured. The results showed that cardiac functions seemed to recover well after ischemia reperfusion injury in the group 2. The values of myocardial dynamics and hemodynamics indexes, including LVSP and ±dp/dt max , in the group 2 were obviously higher than those in the control group after ischemia reperfusion. The content of MDA in myocardial tissue was obviously lower in the group 2 than in the control group after ischemia and reperfusion. There was significant difference between Yixintang group 2 and control group ( P <0 05). It is indicated that 'Yixintang' could protect the myocardium against ischemia reperfusion injury, and it was dose dependent.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第5期447-448,共2页
Medical Journal of Chinese People's Liberation Army
