摘要
目的 :研究活性氧诱导红细胞发生氧化溶血的机制。方法 :用不同浓度的 H2 O2 作用于离体正常红细胞 ,分别用比色法、硫代巴比妥酸荧光法、荧光偏振法测定红细胞溶血度、红细胞膜脂质过氧化物 (L PO)、红细胞膜微粘度。结果 :5 0 ,10 0 ,12 0 mm ol/ L 浓度的 H2 O2 作用于红细胞后 ,均引起红细胞发生氧化溶血 ,红细胞溶血度、红细胞膜 L PO、红细胞膜微粘度均明显增加 ;H2 O2 在 5 0～ 12 0 mm ol· L- 1浓度范围内 ,红细胞溶血度、红细胞膜 L PO、红细胞膜微粘度的增加与 H2 O2 有明显的量效关系 ;红细胞膜微粘度与红细胞溶血度呈明显正相关 ,红细胞膜 L PO与红细胞膜微粘度呈明显正关。结论
Objective:To study the mechanism of erythrocyte oxidative hemolysis caused by reactive oxygen species (ROS) Methods:Different concentrations of H 2O 2 were acting on vitro normal erythrocyte,and the erythrocyte hemolysis degree,erythrocyte membrane lipid peroxide (LPO) and erythrocyte membrane microviscosity (EMMV) were determined in colorimetry method, thiobarbituric acid fluorescence method , and fluorescence polarization method, respectively Results:The erythrocyte oxidative hemolysis happened when erythrocyte were acted upon by H 2O 2 with 50mmol·L -1 , 100mmol·L -1 ,120 mmol·L -1 , the erythrocyte hemolysis degree, erythrocyte membrane LPO and EMMV were increased The effects of the increasing erythrocyte hemolysis degree, erythrocyte membrane LPO and EMMV were obviously dose dependent within extent of 50 mmol·L -1 ～120 mmol·L -1 H 2O 2 There was significant positive correlation between erythrocyte hemolysis degree and EMMV There was also significant positive correlation between erythrocyte membrane LPO and EMMV Conclusion:A part of mechanism of erythrocyte oxidative hemolysis caused by ROS leads to erythrocyte membrane lipid peroxidation, and decrease of erythrocyte membrane fluidity is induced by erythrocyte membrane lipid peroxidation
出处
《广西医科大学学报》
CAS
2001年第2期184-186,共3页
Journal of Guangxi Medical University
关键词
红细胞
氧化溶血
脂质过氧化物
膜流动性
微粘度
活性氧
erythrocyte
oxidative hemolysis
lipid peroxide
membrane fluidity
microviscosity
reactive oxygen species
