摘要
目的 :探讨羊膜对多形核白细胞凋亡的影响 ,同时比较新鲜羊膜和保存羊膜对其影响的差异 ,以初步揭示羊膜移植减轻炎症等作用的有关机制。方法 :先将新鲜羊膜和保存羊膜进行组织块培养 2天后收集条件培养基 ,然后将其加入己分离好的多形核白细胞中继续进行培养 (DMEM培养基作对照组 ) ,培养后 6、 9、 12、 15小时分别应用Dapi荧光染色和流式细胞仪的方法进行凋亡的研究。结果 :每组在各时间点均可见到凋亡细胞 ,凋亡细胞的数量 (流式细胞仪 )随时间的延长而逐渐增多。新鲜羊膜组、保存羊膜组和对照组在 6、 9、 12、 15小时多形核白细胞的凋亡百分比分别为 17 3%、 2 4 4%、 2 9 8%、 37 1% ,16 2 %、 2 0 1%、2 3 7%、 2 7 7%和 10 2 %、 13 7%、 2 1 1%、 2 6 4%。统计学上三组间的差异具有显著性 (组间差值t检验 ,P1<0 0 1,P2 <0 0 1,P3<0 0 1)。结论 :羊膜可以促进多形核白细胞的凋亡 ,进而影响其功能的变化 ,从而减轻炎症和阻止眼表面基质的溶解。新鲜羊膜比保存羊膜更能促进其凋亡 。
Objective:To investigate the effect of fresh and preserved amniotic membrane on polymorphonuclear neutrophils in order to uncerstand the associated anti inflammatory mechanism of amniotic membrane transplantation. Methods:Conditioned medium was collected 48h later when fresh or preserved amnion were cultured in DMEM at 5% CO 2,37℃.Then polymorphonuclear cells were cultured in conditioned culture or DMEM.Fluoreceint microscopy with Dapi staining and cytometry were performed 6h,9h,12h,and 15h later. Results:Apoptotic neutrophils were found in every group at different check point.The percentage of apoptotic cells at 6h,9h,12h,and 15h in fresh amnion group,preserved amnion group and control group was 17 3%,24 4%,29 8%,37 1%,and 16 2%,20 1%,23 7%,27 7%,and 10 2%,13 7%,21 1%,26 4% respectively (t test,P 1<0 01,P 2<0 01 P 3<0 01). Conclusions:Amniotic membrane can fasten apoptosis of polymorphonuclear neutrophils to reduce inflammation and prevent ocular surface collagen from lysis.It seemed that fresh amnion may act stronger than preserved amnion.
出处
《中国实用眼科杂志》
CSCD
北大核心
2003年第1期39-41,共3页
Chinese Journal of Practical Ophthalmology
基金
高校博士点专项科研基金 (A0 32 0 0 1 0 50 80 37)
广东省卫生厅"五个一"工程基金
中山医科大学"2 1 1工程"重点学科建设基金 (A1 32 0 0 1 0 31 )资助
