摘要
目的 构建一种新型压电胰岛素免疫传感器。方法 AT切向、基频 10MHz的石英晶体用金属夹具和乳胶套圈固定形成检测池 ,抗人胰岛素单克隆抗体采用巯基化方法固定在金膜电极表面制成抗体敏感膜 ,构成压电胰岛素免疫传感器。结果 构建的压电胰岛素免疫传感器对胰岛素的响应特性良好 ,其线性检测范围为 2 .5～ 16 0 .0mIU/L ,C肽对胰岛素的检测基本无干扰 ,但胰岛素原与传感器存在轻微的交叉反应 ,传感器可再生后重复使用 5次 ;6 0例临床标本检测结果与放射免疫检测法符合(P >0 .0 5 ) ,两种方法的相关系数为 0 .92。结论 研制的压电胰岛素免疫传感器具有灵敏度高、特异性好、不需标记、操作简单、省时、能实时再现检测和重复使用等优点 ,对比实验表明其检测结果准确 ,能用于临床实验诊断 。
Objective To construct a new type of insulin piezoelectric immunosensor for the determination of human insulin. Methods The sensor pool was consisted of plastic cycle and the crystal that was 10MHz quartz AT cut with gold coated electrodes. The immobilization of the monoclonal antibody of insulin onto the crystal's gold electrode surface was accomplished via Thiolated method. The immunosensor was used in clinical laboratory. Results Experimental results showed that the piezoelectric immunosensor had good response to insulin,the frequency shifts were linearly dependent on insulin concentration in the range of 2.5～160.0mIU/L,other antigens such as C peptide did not interfere with the sensor's response,but proinsulin reacted across the immunosensor lowly. The analytical results given by this approach were in satisfactory agreement with those given by radiometric immunoassay( P >0.05),Correlation coefficient was 0.92. Conclusion Piezoelectric immunosensor for determination of insulin had the features of high sensitivity,high specificity,unnecessary labelling,simple operation,high analysis speed,low cost,real time detection and repeated use,etc. It was suitable for detecting insulin of serum in clinical laboratory.
出处
《重庆医学》
CAS
CSCD
2003年第2期173-175,共3页
Chongqing medicine
基金
国家科技部九五科学仪器攻关项目<科学仪器和实验条件研究与开发>(96 A2 3 0 4 0 4 )
国家高技术计划生物技术领域 (863)青年基金
国家自然科学基金 (39870 831 )
重庆市科委风险投资基金
军队"九五""十五"课题 (98M 0 64
0 1MA1 80
0 1LO0 65)。
