摘要
目的 探讨用PNA作为探针与普通的DNA探针相比对压电传感器基因杂交的优越性。方法 用生物素 亲和素法分别将PNA、DNA两种探针固定在基因传感器的金膜表面 ,分别与完全匹配、1个碱基错配、2个碱基错配的互补靶序列进行杂交 ,观察 2种不同的探针与靶序列反应所引起的频率变化及所用的时间。结果 相对于DNA探针 ,PNA探针识别碱基错配的能力明显高 ,且杂交时间更短。结论 PNA与靶序列DNA结合有高度的特异性 ,选用PNA作探针 ,可提高压电基因传感器检测的特异性。
Objective To investigate the superiority of the gene hybridize of the piezoelectric sensor by comparing the probe of PNA with the ordinary probe of DNA.Methods The probe of PNA and DNA were immobilized on the gold surface with bioth avidin method,and hybridized with target sequence of the complete,one mismatch and two mismatch sequence,respectively.Observe the change of the frequency and the cost time of the reaction of the two different probes.Results The recognizing mismatch base capability of PNA probe was high and the cost time of hybridization was shorter.Conclusion PNA combining with the DNA of target sequence had a highly specificity.Choosing PNA as the probe may improve the determined specificity of piezoelectric gene sensor.
出处
《重庆医学》
CAS
CSCD
2004年第8期1135-1137,共3页
Chongqing medicine
基金
国家 8 63计划重大专项 (2 0 0 2AARZ2 0 2 3)
国家"九五"科技攻关项目 (96 A2 3 0 4 0 4 )
国家自然科学基金 (30 2 70 388)
军队"九五"课题 (98MO94)
军队"十五"课题 (0 1MA1 80
0 1LO65)
重庆市应用基础研究项目 (2 0 0 2 1 0 78)
重
关键词
肽核酸
核酸杂交
传感器
压电晶体
peptide nucleic acid(PNA)
nucleotide hybridization
sensor
piezoelectric crystal
