摘要
花生种子中富含蛋白质、多糖和酚类化合物,从而增加了从中提取RNA的难度。作者通过实验对几种方法做了比较,并对其中的异硫氰酸胍方法稍做改变,结果得到的RNA产率较高,质量好,符合分子生物学实验要求。
Peanut seeds are rich in lipid, protein, polysaccharide and phenolic compounds, which increases the difficulties in isolating RNA from them. We compared several methods through experiments and adopted a slightly motified guanidine thiocyanate method. The yield and quality of RNA extracted using this method were high and fit for the standard of many common molecular biology experiments.
出处
《花生学报》
2002年第4期24-26,共3页
Journal of Peanut Science
基金
中国农科院油料作物研究所重点开放实验室课题部分实验
关键词
花生种子
RNA
提取
改进方法
peanut seed
guanidine thiocyanate
RNA extraction
