摘要
分子印迹是一项模拟靶向生物分子特异性识别能力的仿生分子识别技术,因其识别选择性好、制备简单、价格低廉等优势而被广泛应用.发展至今,已有多种先进的印迹方法被报道,包括表面印迹、表位印迹、乳液印迹等,克服了传统本体印迹方法中存在的许多问题,也使分子印迹技术在许多应用领域成为理想的抗体替代品.由于糖抗体极为稀缺,因此发展出针对糖类化合物的分子印迹技术显得尤为重要,而硼亲和可控定向表面分子印迹技术的提出为解决该问题提供了极有力的技术支持.该技术是一项可控、通用、高效的分子印迹技术,几乎适用于各种类型的基质材料和所有糖类化合物的印迹.它的提出不仅进一步丰富了分子印迹技术的应用范围,更是从方法学上将分子印迹技术推向了一个新的高度,因此具有重要的现实与科学意义.本文介绍了硼亲和可控定向表面分子印迹技术的主要制备方法、原理、应用及其最新研究进展,提出了该技术目前存在的若干问题并展望其未来可能的发展方向.
Biomolecules,like antibody,aptamer and lectin,are still the main force for applications involved in selective separation and recognition.However,these biomolecules are usually suffered from many inevitable problems,such as hard to prepare,high cost,poor stability,as well as limited quantities.Molecular imprinting technique(MIT)as a robust biomimetic molecular recognition strategy to mimic the targeting ability of antibody and enzyme plays a significant role in many areas,including chemical separation,biosensing,catalysis,disease diagnosis,etc.Scientists have pay a lot of attention to developing advanced imprinting approaches to enable MIT adapt many more possible applications.Several user-friendly molecular imprinting methods,such as surface imprinting,epitope imprinting and emulsion imprinting,are hence developed to meet the high analytical requirements.Even so,facile MITs with wide-range versatility and high imprinting efficiency are extremely limited,which restrict the applicability of MIT.Boronate affinity-based controllable oriented surface imprinting(BACOSI)allows for easy and efficient preparation of molecularly imprinted polymers specific to saccharide compounds,which covering glycoproteins,glycopeptides,glycans and monosaccharides.In this paper,the principle,preparation process,applications and recent developments of BACOSI are briefly introduced.Three steps,namely template immobilization,oriented imprinting and template removal,are included in this approach.Template molecules(glycoprotein,glycopeptide,glycan or monosaccharide)immobilized on the surface of boronic-acid-functionalized substrate by virtue of boronate affinity interaction,followed by controllable selfpolymerization of biocompatible monomer(s)to form an imprinting layer on the template-anchored substrate with tailored thickness in terms of molecule size of templates,and then the templates are effectively removed under suitable conditions(weak acidic solutions with or without surfactant are often utilized for templates removal).Imprinting in
作者
王双寿
卢小媚
程婕
葛琼琼
Shuangshou Wang;Xiaomei Lu;Jie Cheng;Qiongqiong Ge(School of Chemistry and Chemical Engineering,Anhui University of Technology,Ma’anshan 243032,China;Department of Cardiologie,Nanjing BenQ Medical Center,the Affiliated BenQ Hospital of Nanjing Medical University,Nanjing 210019,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2019年第13期1340-1351,共12页
Chinese Science Bulletin
基金
安徽工业大学人才项目(DT18100003)
安徽工业大学校青年基金(RD18100229)
南京大学生命分析化学国家重点实验室开放研究基金(SKLACLS1801)资助
关键词
硼亲和
分子印迹
仿生分子识别
细胞成像
糖蛋白
癌症
boronate affinity
molecular imprinting
biomimetic molecular recognition
cell imaging
glycoprotein
cancer
