摘要
以DNA折纸为模板的荧光阵列具有精准的结构可编程性以及可定量编程的光谱特征.利用DNA折纸灵活的设计性,多种维度不同模式的荧光阵列构建方案相继被提出,并在生物传感、超分辨成像、新型荧光探针设计等领域得到了应用.本文简要介绍了DNA折纸技术,对不同维度DNA折纸上的荧光阵列的构建和应用做了综述,并展望了基于DNA折纸的荧光阵列的发展趋势和应用前景.
Organic fluorescent molecules with excellent optical properties such as small size and high brightness,are widely used in the field of bioimaging and target molecule detection.Compared to monodispersed fluorescent molecules,fluorescence arrays provide spatial controllability and quantitative programmability for patterning fluorescent molecules,which open new possibilities for expanding the applications of fluorescent molecules.The development of DNA origami techniques allows sub-nanometer addressing capabilities,which is the structural basis for organizing molecules at designed positions.DNA single strands form duplex through complementary pairing,which provides simplicity,efficiency and high orthogonality for construction and diverse programming of fluorescence arrays on DNA origami.Meanwhile,the flexible design of DNA origami provides abundant templates for one-dimensional,two-dimensional and three-dimensional fluorescence arrays of different shapes,making it widely used as a platform for building nanoscale spatial molecular arrays.The characterization of topography on DNA origami tremendously relies on atomic force microscopy(AFM).However,fluorescent molecules on DNA origami usually do not introduce significant topographical change,therefore,cannot be observed well using AFM.Recently developed DNA-PAINT(point accumulation for imaging in nanoscale topography)super-resolution imaging method is based on transient interactions between DNA oligos,which realize the characterization of nanoscale structures by fluorescent imaging.It is a new routine for the characterization of the fluorescence arrays on origami.The development of detectors such as EMCCD and CMOS also provides a higher time resolution for the detection of fluorescent signals,facilitating more accurate dynamic study of interaction between molecules In this review,we summarize recent research progress on the DNA origami-based fluorescence arrays.We introduced the design and assembly principle of DNA origami as template for patterning fluorescent dyes,and
作者
王飞
王丽华
樊春海
李茜
Fei Wang;Lihua Wang;Chunhai Fan;Qian Li(School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2019年第10期989-1000,共12页
Chinese Science Bulletin
基金
国家自然科学基金(41474032
21505148)资助
