摘要
核酸适配体是一类能够与靶标物高特异性地结合的寡核苷酸序列,可作用于金属离子、小分子化合物、蛋白质及细胞等.由于对靶标物具有高选择性,核酸适配体可赋予递送体系靶向特异性,同时增加递送药物及成像试剂在肿瘤组织内的富集,在分子诊断和体内靶向治疗等生物医学领域具有很大的应用潜能.本文基于聚丙烯酸(PAA)修饰的NaYF4:Yb/Er/Nd@NaYF4:Nd上转换纳米颗粒(UCNPs-PAA),将其封装于AS1411核酸适配体修饰的脂质体(Apt-Lip)中,构建了一种近红外(NIR)光激发的光学成像及靶向识别的UCNPs@Apt-Lip多功能纳米平台.透射电子显微镜、水合粒径、Zeta电势及共聚焦实验图表明成功制备了具有独特的核@空腔@壳构造的纳米体系.流式细胞仪和靶向细胞荧光共聚焦成像实验结果表明UCNPs@Apt-Lip材料对人乳腺癌细胞具有很强的靶向识别及荧光成像的能力.该多功能纳米平台具有上转换发光成像的可动核心,中空内腔和靶向壳层等独特性能,有望推动其在光催化纳米反应器、药物输送、肿瘤治疗等领域的发展.
During tumor treatment,effective imaging diagnosis can monitor the biological distribution of nanomaterials in vivo,so as to reveal the therapeutic effect of nanomaterials and provide comprehensive information of tumor tissue.In conventional cancer therapy,nanomaterials that fail to distinguish between tumor cells and normal cells often lead to adverse side effects and higher systemic toxicity.Therefore,more and more attention has been paid to the development of multifunctional nano-platform for a variety of biomedical applications.Aptamers are single-stranded oligonucleotide that can specifically recognize their substrates,such as metal ions,small molecule,proteins and even cells.Owing to their high specificity,aptamers can endow the delivery system with robust targeting ability and enhance the enrichment of drugs and imaging agents in tumor tissues,holding great promise in molecular diagnosis and cancer therapy.Here,we designed a NIR-activated optical imaging and target-recognition multifunctional nanoplatforms by the capsulation of poly(acrylic acid)(PAA)coated NaY F4:Yb/Er/Nd@NaYF4:Nd upconversion nanoparticles(UCNPs-PAA)within aptamer-guided liposome(Apt-Lip)to form UCNPs@Apt-Lip.Transmission electron microscopy(TEM)images showed that the composite nanomaterials had a uniform spherical structure,and the UCNPs nanoparticles were successfully loaded into the liposome modified by AS1411 aptamer,and the monodispersity of nanoparticles was good.The Dynamic light scattering(DLS)detected that the hydrated particle size of UCNPs@Apt-Lip modified by AS1411 aptamer changed from 165.6 nm to 209.5 nm,and the zeta potential(-22.7 mV)was lower than UCNPs@Lip(-20.5 mV),which further confirmed that the AS1411 aptamer was successfully connected to the surface of UCNPs@lip.Flow cytometry and targeted cell fluorescence imaging experiments show that UCNPs@Apt-Lip nanostructure has strong ability of target recognition and fluorescence imaging to MCF-7 cells.With the unique properties of movable cores,interstitial hollow spaces,a
作者
肖射华
梁玲
李丹
苏元野
袁荃
Shehua Xiao;Ling Liang;Dan Li;Yuanye Su;Quan Yuan(College of Life Sciences,Hunan University,Changsha 410082,China;Molecular Science and Biomedicine Laboratory,Institute of Chemical Biology and Nanomedicine,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2019年第10期1067-1075,共9页
Chinese Science Bulletin
基金
国家自然科学基金(21675120)资助
