摘要
以余甘子叶片为材料,研究了余甘子基因组DNA提取方法及RAPD反应条件.结果表明,采用改良的SDS方法,提取的DNA质量较高,适宜于RAPD-PCR分析.在25μL反应体积中,RAPD分析的优化反应体系为:0.6mmol·L-1Mg2+、500-600μmol·L-1dNTP、300nmol·L-1随机引物、25ngDNA、1.00-1.25UTaqDNA聚合酶.
The method of genomic DNA extraction and the optimization of RAPD analytic conditions were studied in Phyllanthus emblica L. The results showed that high grade genomic DNA was obtained by the revised SDS method. The optimal PCR system for RAPD analysis was as follows: 0.6 mmol·L-1 Mg2+, 500-600 μmol·L-1 dNTP, 300 nmol·L-1 random primer, 25 ng DNA template, 1.00-1.25 U Taq polymerase in 25 μL reaction solution.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2003年第1期89-92,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
