摘要
目的:探讨感染HCV1b型慢型丙型肝炎患者HCVHVR1区准种的复杂性与干扰素疗效的关系,以便对干扰素治疗疗效进行预测.方法:应用IFN治疗的慢性丙型肝炎患者共20例,于治疗前、治疗后3mo、6mo留取血清,-70℃冻存待测.治疗方案为:干扰能3mu,3次/wk,肌肉注射,共24wk.HCVHVR1准种的检测采用单链构象多态性聚合酶链反应法(SSCP法).结果:IFN治疗前,35%(7/20)的患者表现为SSCP低复杂性(SSCP条带数≤3),65%(13/20)表现为高复杂性(SSCP条带数>3).治疗3mo后,4例患者HCVRNA阴转,均发生在低复杂性组,其余大部分患者SSCP条带数减少1-2条带.治疗6mo时,7例患者HCVRNA阴转,仅2例发生在高复杂性组.治疗结束时13例(65%)无应答患者中,8例患者SSCP条带数均较治疗前减少,2例无变化,1例恢复到治疗前水平,2例较治疗前升高.结论:HCVHVR1区准种的复杂性可以对IFN治疗进行疗效预测,治疗前准种数少者可预测对IFN治疗有较好的应答.
AIM:To evaluate the correlation between the complexity of HCV HVR1 quasi-species and their response to IFN therapy in patients with chronic hepatitis C. METHODS:Twenty patients with chronic hepatitis C received IFN therapy (3mu/day, three times/week for 24 weeks). Serum quasispecies complexity of HVR1 was analyzed by polymerase chain reaction mediated singl-strand conformation polymorphism (SSCP) before and 3 months,6 months post- therapy, respectively. RESULTS:Of 20 patients,7 had low level of complexity (SSCP bands ≤ 3),13 had high level of complexity (SSCP bands >3). The rate of HCV RNA negative in low level complexity group was higher than that in high level complexity group.Patients with low level of complexity prior to therapy had good responsive to IFN;Serum HCV RNA in some patients did not convert to negative after IFN therapy,however, the number of SSCP bands decreased gradually. CONCLUSION:HCV HVR1 quasi-species complexity is a predictive factor in response to IFN therapy, a statistically significant correlation between high level of complexity of HCV HVR1 quasi-species and lacking of response to IFN therapy in patients with chronic hepatitis C was found.
出处
《世界华人消化杂志》
CAS
2003年第2期182-184,共3页
World Chinese Journal of Digestology
