摘要
目的:研究HCVHVR1准种异质性与干扰素疗效以及与HCVRNA载量之间的关系,为临床选择应用干扰素抗病毒治疗适应症及预测疗效提供理论依据。方法:采用核苷酸序列测定法进行HCV基因分型;分别采用单链构象多态性聚合酶链反应法(SSCP)及克隆测序法进行HVR1准种异质性检测;采用荧光特异性PCR法进行HCVRNA载量检测。结果:14例1b型慢性丙肝患者干扰素治疗前5例为SSCP低复杂性(SSCP条带数≤3),9例为高复杂性(SSCP条带数>3),干扰素治疗应答组SSCP条带数明显少于无应答组。1b型患者中无应答组HVR1变异株的数目(准种数)和基因的差异性(每个基因位点的平均变异率)均明显高于应答组。HVR1准种异质性程度与HCVRNA含量无正相关关系。结论:感染HCV基因型1b型的慢性丙型肝炎患者HVR1准种异质性程度越高对干扰素无应答的可能性越大。HVR1准种的复杂性程度与HCVRNA含量无关,是预测干扰素疗效的一个独立因素。
Objective： To study the relationship between the heterogeneity of HCV HVR1 quasispeeies and the effectiveness of IFN therapy and HCV RNA level.Methods：The HVR1 quasispeeies heterogeneity was detected by polymerase chain reaction-mediated single-strand conformation polymorphism（SSCP） and cloning and sequencing. HCV RNA level was detected using the method of specified fluorogence probe PCR.Results：Among 14 patients infected with HCV 1b,5 showed low complexity（SSCP band numbers≤3） ,9 showed high complexity（SSCP band numbers〉3） .The SSCP bands were more in non-response group than those in response group.The numbers and the nueleotide diversity of HVR1 quasispeeies in the non-response of group were significantly higher than those in the response of group.There were no correlation between the complexity of HVR1 quasispecies and HCV RNA level. Conclusion：The higher heterogeneity of HVR1 quasispeeies may lead to non-response to IF therapy. The complexity of HVR1 quasispecies was an independent predicting factor for the effectiveness of IFN therapy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2005年第9期714-716,F0004,共4页
Chinese Journal of Immunology
基金
辽宁省教育厅资助项目(202013120)
