摘要
目的 :检测吗啡依赖大鼠血清肝功能生化指标及核苷酸代谢参数的变化。方法 :Wistar大鼠 3 5只 ,随机分成 5组 ,每组 7只。第 1组为对照组 ,给予 0 .9%氯化钠注射液 ;第 2组给予盐酸吗啡 2 0～ 95mg·kg 1 ·d 1 ,分 2次 ,ip ,共7d ;第 3组在给予盐酸吗啡的同时 ,给予嘌呤碱基混悬液 10 0mg·kg 1 ·d 1 ,ig ,第 8天给予盐酸纳洛酮 4mg·kg 1 ,ip ,观察戒断体征 ;第 4组用嘌呤核苷混悬液替代嘌呤碱基混悬液 ,其余同第 3组 ;第 5组给予盐酸吗啡和盐酸纳洛酮 ,用量和用法同第 3组。采用Beckman丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)及尿酸检测试剂盒测定相应指标。结果 :发现了吗啡依赖大鼠血清尿酸水平升高 ,ALT及AST酶活性升高。结论 :吗啡可引起组织细胞嘌呤核苷酸分解代谢增强 。
Objective:To assay the changes in the biochemical indices of liver function as well as paramenters of nucleotide metabolism in the serum of morphine dependent rats. Methods:35 Wistar rats were randomly divided into 5 groups with 7 animals in each group.①Control group: Rats were given each an IP injection of normal saline b.i.d., for 7 days. ②Morphine dependent: Rats were given each increasing dosages of morphine (20～90 mg·kg 1 ·d 1 ,IP, in 2 divided doses) for 7 days. ③Morphine dependent+purine bases+naloxone:Rats were given injections of morphine as in group②+purine bases (100 mg·kg 1 ·d 1 )fed by gastric tube for 7 days, +IP injection of naloxone (4 mg·kg 1 ) on the 8th day and withdrawal symptoms were kept under observation. ④ Morphine dependent+purine nucleosides+naloxone:Rats were treated in the same manner as in group ③ except that purine nucleosides were used instead of purine bases. ⑤Morphine dependent+withdrawal with naloxone: Rats were given morphine as in group②, followed by IP injection of naloxone as in group③. Beckman's kits were used for the determination of uric acid and ALT/AST in the serum. Results:Serum uric acid as well as activities of ALT and AST were found to increase in morphine dependent rats. Conclusion: Morphine dependence was shown to enhance catabolism of purine nucleotides and cause injury of the liver.
出处
《医药导报》
CAS
2003年第4期224-226,共3页
Herald of Medicine
