摘要
利用白玉蜗牛的内脏废弃物,以蜗牛纤维素酶为主要提取酶类,采用正交实验的方法初步探讨了蜗牛酶的提取工艺条件。研究结果表明:蜗牛纤维素酶的提取时间约为10h、pH为5.0、提取温度为0℃,盐析饱和度为70％,得到纤维素粗品的最高酶活力为1.402U·ml^(-1)。
This paper makes a research on the technical conditions about how to abstract cellulase from Helix pomatia pomatia. The optimum conditions were studied through orthogonal experiment. The results manifest that the best abstraction pH and temperature were 5.0 and 0℃ respectively, and the optimum time is about 10 hours. On the basis of the above conditions we abstracted primary product of cellulase through partition chromatography and found the most active unity of cellulase is 1.402 U · ml-1.
出处
《生态科学》
CSCD
2002年第4期327-329,共3页
Ecological Science
关键词
白玉蜗牛
纤维素酶
提取工艺
Helix pomatia , Cellulose, Extractive technology
