摘要
观察不同参数(频率、脉宽、峰值)组合的陡脉冲电场对恶性肿瘤细胞的杀伤效应。将癌细胞悬液3 ml盛于玻璃瓶中,每次施加不同参数组合的电脉冲20 min,用MTT法测细胞死亡率,并制作生长曲线;观察细胞的超微结构变化。结果发现:不同参数组合的陡脉冲对癌细胞的杀伤有较大影响,随着参数值的增大,细胞死亡率上升,细胞的增殖受到抑制;实验组的细胞肿胀,溶解性坏死,仅留细胞轮廓,细胞膜完整性破坏,线粒体空化、肿胀。
In order to observe the lethal effects of pulsed electric fields (PEFs) of different parame-ter combinations (frequency, peak value of voltage, width of pulse) on malignant tumor cell, every PEF was applied to malignant tumor cell suspension (3 ml) in the test bottle for 20 min. The MTT method was used to test the mortality ratio of tumor cell and growth curve was plotted. The ultrastructural varia-tions of treated cells were inspected and compared with the untreated cells. The results showed that PEFs of different combinations had great influence on tumor cell. With the increasing of parameter value, the mortality ratio of tumor cell was rising and cell proliferation was inhibited. The treated cell was swollen and the membrane was destroyed. The mitochondria was also cavitated and swollen.
出处
《生物物理学报》
CAS
CSCD
北大核心
2002年第4期474-477,共4页
Acta Biophysica Sinica
基金
国家自然科学基金项目(50077027)
关键词
陡脉冲电场
恶性肿瘤
细胞膜
不可逆性电击穿
Pulsed electric fields (PEFs)
Malignant tumor
Cell membrane
Irreversible electrical breakdown (IREB)
