摘要
目的 :研究低能陡脉冲对肿瘤细胞核DNA的损伤及诱导凋亡作用 ,分析其可能的机制及对电化学治疗潜在的影响。方法 :观察频率、脉宽、电压峰值固定 ,上升时间递增的低能陡脉冲对人卵巢癌细胞SKOV 3的损伤效应 ,用彗星试验检测细胞核DNA的损伤 ,计算拖尾率及拖尾长度以评估DNA损伤的程度 ,联合DNA片段化分析检测染色体断裂情况及电镜观察超微结构的改变。结果 :细胞核DNA拖尾率及拖尾长度与外加脉冲的上升时间有一定的量 效关系 ,DNA损伤、片断化程度随外加脉冲上升时间的增加而加重 ,电镜下出现典型的凋亡样改变。结论 :低能陡脉冲对瘤细胞核DNA有确切的损伤效应 ,可诱导瘤细胞凋亡。
Abstract Objective:The aim of our study was to determine the damage effects of low energy steep pulse (LESP) on nuclei DNA of human ovarian cancer cell line,SKOV3 cell lines,to analysis its possible mechanisms and implications for electrochemotherapy.Methods:LESP with fixed frequency,width,peak value of voltage and gradually increased rise time of LESP were applied to SKOV3 cells.Comet assay was used to detect DNA damage of SKOV3,rate of tailedDNA and taillength of DNA migration were calculated for evaluation the degree of DNA damage,combination with DNA fragmentation analysis examined chromosomes breaks,and electron microscopy was used to detect ultrastructural changes.Results:A certain doseeffect relationship was observed between rate of tailedDNA,taillength and rise time of LESP.The degree of DNA damage and fragmentation positively correlated with rise time of LESP.A typical apoptosis also occurred under electron microscope.Conclusions:Our results showed that LESP has a certain damage effect on nuclei DNA of SKOV3 cell lines,also can induce apoptosis in these cells.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2004年第6期343-345,i014,共4页
Journal of Practical Obstetrics and Gynecology
基金
国家自然科学基金资助项目 (30 371 61 9)
关键词
低能陡脉冲
彗星试验
DNA损伤
凋亡
[Key words] Low energy steep pulse(LESP)
Comet assay
DNA damage
Apoptosis
