摘要
由双拷贝CaMV35S启动子驱动担子菌灰树花 (Grifolafrondosa)的海藻糖合酶基因 (TSase)构建植物表达载体 pBBBT ,通过三亲交配法将 pBBBT导入根癌农杆菌EHA10 5菌株 ,经根癌农杆菌介导转化甘蔗 (Saccha rumhybrid)栽培品种 ,以增强甘蔗的抗旱能力。结果表明 ,甘蔗胚性愈伤组织对EHA10 5菌株敏感 ,甘蔗外植体开始大量形成胚性愈伤组织时是感染的适宜时期 ,用膦丝菌素 (PPT)筛选 ,抗性植株发生频率平均为 4 .5 %。经PCR及dot Southern检测证明 ,TSase已经整合到甘蔗基因组中。部分转化植株根叶畸形、株型异常、生长缓慢。移栽到含PEG80 0 0 17.4 % (w/v)的MS培养基后 。
Trehalose synthase gene (TSase) from Grifola frondosa was introduced into sugarcane (Saccharum hybrid) by Agrobacterium mediated to improve drought tolerance of sugarcane. The transformation system demonstrated that embryogenic callus of sugarcane was sensitive to A. tumefaciens EHA105 strain. Transformation of callus in 3 weeks after incubation produced the highest frequency of PPT resistant plant, which reached 4.5% in average. The transgene was confirmed by PCR and dot Southern analysis. Some plants of transgenic sugarcane showed multiple phenotypic alterations. After transplantation onto MS medium contained 17.4 % PEG 8000 the transgenic plants demonstrated their improvement in tolerance to osmotic stress.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第2期140-146,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目 ( 3 0 160 0 45 )
