摘要
将抗虫ＰＩ基因构建到ｐＩＧ１２１Ｈｍ质粒的Ｔ－ＤＮＡ上，利用农杆菌ＬＢＡ４４０４介导转化烟草，获再生植株．经ＧＵＳ检测和Ｄｏｔｂｌｏｔ分析，证实外源基因已插入植物细胞基因组中，再生植株的转化率为６４．３％．
Plant expression vector with resistance insect gene (PI) has been obtained by inserting PI gene into the T DNA of binary pIG121Hm Vector Tobacco was transformed with A tumefaciens LBA4404 containing PI gene The Gus assay and Dot blot analysis from regenerated plants indicate that PI gene has been introduced into tobacco genome by T DNA. 64 3% transgenic plants was obtained
关键词
根癌农杆菌
烟草
PI基因
转基因植株
Agrobacterium tumefaciens
tobacco
PI gene
transgenic plants
