摘要
目的:研究锌指蛋白ZNF222在滋养层细胞增殖、迁移及侵袭中的功能。方法:免疫组织化学法检测ZNF222在人妊娠早期绒毛、蜕膜组织及非妊娠状态下子宫内膜组织中的表达。小干扰RNA敲降ZNF222的表达。CCK-8法检测细胞增殖能力。Transwell小室检测细胞的迁移和侵袭能力。结果:锌指蛋白ZNF222在人妊娠早期绒毛中高表达、蜕膜组织中表达较弱,非妊娠状态子宫内膜组织表达非常弱。在人滋养层细胞系HTR8/SVneo中,敲降ZNF222后,细胞增殖能力无显著变化(ZNF222 siRNA-1,P=0.610; ZNF222 siRNA-2,P=0.277),但细胞迁移(ZNF222 siRNA-1,P=0.0265;ZNF222 siRNA-2,P=0.00586)和侵袭(ZNF222 siRNA-1,P=0.0327;ZNF222 siRNA-2,P=0.0412)能力显著减弱。结论:锌指蛋白ZNF222调控着人滋养层细胞的迁移和侵袭,推测ZNF222在人类胎盘形成过程中发挥重要作用。
Objective:To study the function of zinc finger protein ZNF222 in trophoblast cell proliferation,migration and invasion.Methods:Immunohistochemistry was used to detect the expression of ZNF222 in villiand decidua tissue of women with the first trimester of pregnancy and in endometrium tissue of non-pregnant women.After the knockdown of ZNF222 was done by small interfering RNA of human trophoblast cell,the cell proliferation was tested by CCK-8.The cell migration and invasion were tested by transwell.Results:ZNF222 expressed highly in villi tissue,but expressed weakly in decidua tissue of women with the first trimester of pregnancy,and ZNF222 expressed very weakly in the endometrial tissue of non-pregnant women.After the knockdown of ZNF222 in human trophoblast cell line HTR8/SVneo,there was no significant change in cell proliferation(ZNF222 siRNA-1,P>0.05,P=0.610;ZNF222 siRNA-2,P>0.05,P=0.277),but cell migration and invasion had reduced significantly(migration:ZNF222 siRNA-1,P<0.05,P=0.0265,ZNF222 siRNA-2,P<0.01,P=0.00586;invasion:ZNF222 siRNA-1,P<0.05,P=0.0327,ZNF222 siRNA-2,P<0.05,P=0.0412).Conclusion:ZNF222 can regulate the migration and invasion of human trophoblast cells.It is speculated that ZNF222 may play an important role in human placenta formation.
作者
刘建兵
赵皓琦
周芳
陈西华
郝建卿
徐祥波
贺斌
王介东
LIU Jianbing;ZHAO Haoqi;ZHOU Fang;CHEN Xihua;HAO Jianqing;XU Xiangbo;HE Bin;WANG Jiedong(School of Basic Medical Sciences,Shanxi Medical University,Taiyuan,030001;National Research Institute for Family Planning)
出处
《中国计划生育学杂志》
2019年第3期281-285,294,共6页
Chinese Journal of Family Planning
基金
北京市自然科学基金(7152115)
国家自然科学基金(81571410)
