摘要
目的探讨原癌基因c-fos的表达和脑震荡性损伤的关系,同时寻找脑震荡后法医病理学的诊断依据。方法55只实验大鼠随机分为脑震荡组和对照组。用免疫组织化学SABC法观察大鼠脑震荡后脑内C-FOS蛋白表达的变化规律。结果对照组大鼠未见C-FOS蛋白的表达。然而脑震荡组损伤后15min即可在神经细胞观察到C-FOS蛋白的表达,随着损伤后经过时间的延长,表达C-FOS蛋白的细胞数及范围逐渐扩大,损伤后6h表达达高峰,直至损伤后96h均有大量C-FOS蛋白表达。结论 c-fos原癌基因的检测可成为诊断脑震荡和推断脑震荡后经过时间的一项敏感指标。
Objective To study the relationship between expression of C-FOS protein and brain concussion and find a sensitive marker of diagnosis of the brain concussion. Methods Fifty-five rats were randomly divided into brain concussion groups and control group. The expression of C-FOS protein was microscopically observed by immunohistochemical method. Results There were negative expression of C-FOS protein in control group. In brain concussion group, however, positive expression of C-FOS protein in some neurons was seen at 15min after brain concussion, and reach to the peak at 6h after brain concussion, then decreased gradually. Conclusion These findings suggest that detection of C-FOS protein could be an index of diagnosis of brain concussion and a sensitive marker of timing of injury after brain concussion.
出处
《法医学杂志》
CAS
CSCD
2003年第1期8-9,共2页
Journal of Forensic Medicine
基金
安徽省自然科学基金项目(No.2002kj313)
