摘要
目的研究脑震荡后脑组织损伤修复相关因子表达变化,为推断脑损伤时间提供法医学依据。方法建立SD大鼠脑震荡伤模型,提取脑组织行常规HE染色及热休克蛋白70(heat shock protein 70,HSP70)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和转化生长因子β1(transforming growth factor beta1,TGF-β1)免疫组织化学染色,在光学显微镜下观察并进行图像分析及相应统计学处理。结果伤后30min,在大脑皮质、海马区见少量呈弥散性分布的HSP70免疫组化染色阳性细胞,12h达高峰,伤后1d阳性细胞数量开始减少;伤后3h,在大脑皮质、海马区bFGF免疫组化染色阳性细胞开始增多,至12h时达高峰,然后开始下降;伤后6h～1d,在大脑皮质、海马区TGF-β1免疫组化染色阳性细胞逐渐增多,至3d时达高峰,然后开始下降。结论HSP70、bFGF、TGF-β1在脑震荡后脑组织中表达呈时序性改变。多指标综合应用为法医学损伤时间的推断提供了全新的视角。
Objective To study the changes of expression of relevant factors in rat Dram atter concussion injury and to provide scientific basis for forensic estimation of brain injury interval. Methods Brain tissues were sampled from the established SD rat animal model of brain concussion, routinely processed and stained with HE and immunohistochemically stained with antibodies directed against heat shock protein 70 （HSP70）, transforming growth factor beta 1（TGF-β1） and basic fibroblast growth factor（bFGF）. The sections were examined under light microscope with IMAGE analytical system and homologous statistical analysis. Results The expression of HSP 70 was observed in 30 minutes after brain injury. The amount of neurons expressing HSP 70 increased gradually, reached its peak at 12 hours and then declined at 24 hours after brain injury. The expression of bFGF was observed 3 hours after injury in brain stem, reached its peak at 12 hours, and then declined. The expression of TGF-β1 was detected 6-24 hours after brain injury, remained at its peak up to 3 days. Conclusion Brain injury can induce a chronological expression of HSP 70, bFGF and TGF-β1. The results can be a potential for estimating the age of brain injury using several markers.
出处
《法医学杂志》
CAS
CSCD
2009年第4期249-253,共5页
Journal of Forensic Medicine
