摘要
目的研究大鼠骨骼肌挫伤模型细胞内碱性成纤维细胞生长因子(bFGF)和c-Fos阳性细胞率比值的变化与损伤时间的关系,为骨骼肌损伤时间的分析提供新的依据。方法选用健康成年SD大鼠65只,体重180~200g。依据处死时间的不同,随机分为12个实验组和1个对照组,每组5只。实验组大鼠于伤后0.5h、1h、3h、6h、12h、1d、2d、3d、4d、7d、10d、14d处死,制作大鼠骨骼肌挫伤模型;对照组大鼠常规饲养24h后处死。分别实验组大鼠取挫伤处骨骼肌及对照组大鼠正常骨骼肌作bFGF和c-Fos免疫组化染色,分析bFGF和c-Fos的表达情况。结果当骨骼肌损伤时间在0.5~3h时,bFGF和c-Fos的阳性细胞率比值为0,且c-Fos阳性细胞率升高;当骨骼肌损伤时间在3h^1d时,bFGF和c-Fos的阳性细胞率比值在0~1.22,且c-Fos阳性细胞率高于bFGF;当骨骼肌损伤时间>1d时,bFGF和c-Fos的阳性细胞率比值>1.22并持续升高。结论 bFGF和c-Fos阳性细胞率比值所呈现的单调性时序性改变,表明采用两种表达时间存在先后差异的生化指标,通过二者阳性细胞率比值的变化规律并结合二者阳性细胞率的变化情况,可推断大鼠骨骼肌挫伤的损伤时间。
Objectives To study the correlation between the ratio of basic fibroblast growth factor （bFGF） to c Fos positive cells in skeletal muscle contusion and the injury time in rats, so as to provide new references for the analysis and diagnosis of the injury time of skeletal muscle. Methods A total of 65 heathy male SD rats weighing from 180 g to 200 g were selected and divided into 12 experiment group and 1 control group according to the execution time, and each group included 5 rats. The rats were executed 0.5 h, 1 h, 3 h, 6 h, 12 h, 1 d, 2 d, 3 d, 4 d, 7 d, 10 d and 14 d after skeletal muscle contusion respectively to establish the models. The rats in the control group were executed after being fed conventionally for 24 h. The expression of bFGF and c Fos was analyzed in the injured skeletal muscle of the experiment group and the normal skeletal muscle of the control group by immunohistochemical staining. Results When the injury time was between 0.5 h and 3 h, the ratio of bFGF/c Fos remained 0 and the numbers of c Fos positive cells were increased. When the injury time was between 3 h and 1 d, the ratio of bFGF/c Fos was between 0 and 1. 22 and the ratio of c Fos positive cells were higher than that of bFGF positive cells. When the injury time was more than 1 d, the ratio of bFGF/c Fos was greater than 1.22 and kept rising. Conclusion The ratio of bFGF to c Fos positive cells shows monotonic temporal changes, which means that two biochemical indicators with different expression time could be used to infer the injury time of skeletal muscle by observing the changes of bFGF and c Fos positive cells and their ratio.
作者
李林峰
曾达
林伟
李雪榕
柳丹凤
田甜
刘敏
Li Linfeng;Zeng Da;Lin Wei;Li Xuerong;Liu Danfeng;Tian Tian;Liu Min(West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu 610041,China;Guangdong Nantian Institute of Forensic Science,Shenzhen 518045,China)
出处
《成都医学院学报》
CAS
2018年第4期405-408,共4页
Journal of Chengdu Medical College
