摘要
目的 实验用小鼠的遗传质量分析和品系鉴定。方法 应用AFLP技术对 10个品系小鼠进行分析。结果 共应用 2 5条单酶切引物、2 5对双酶切引物进行检测 ,其中 17条单酶切AFLP引物、2 0对双酶切AFLP引物检测到 10个品系小鼠的多态性变化。单酶切AFLP共扩出 2 5 1条带 ,片段大小在 10 0～ 2 0 0 0bp ,共得到 89个多态位点 ,占总扩增带的3 5 5 %。双酶切AFLP在 5 0～ 60 0bp扩增出清晰条带 ,统计 15 0 7条带 ,共得到 3 78个多态位点 ,占总扩增带的 2 5 1%。对多态性片段进行统计 ,计算出不同品系间的相似指数和遗传距离指数。结果表明昆明鼠 (KM )与TA2、BALB c、BALB c nu关系较近 ;所有品系间关系最近的两品系为BALB c和BALB c nu ;关系较远的是DBA 2与T73 9、615、C5 7BL 6J ,与小鼠品系来源及培育史相符。结论 通过筛选获得特异性AFLP标记 ,可用以区分遗传背景或外观形态很相似的品系。
Objective To analyze the genetic quality and to determine the strain of laboratory mice. Methods Ten strains of mice were analyzed by using AFLP method. Results Polymorphism was detected in 10 strains of mice by 17 single enzyme primers and 20 pairs of double enzyme primers amplification. A total of 251 bands were shown by single enzyme AFLP in agarose gel with the size of the bands ranging from 100 bp to 2 000 bp and 89 polymorphic loci were detected. A total of 1507 clear bands between 50 bp and 600 bp were shown by double enzyme AFLP and 378 polymorphic loci were detected. Through statistical analysis, we calculated the similar index and genetic distance index. Our results showed BALB/c and BALB/c nu had the closest relationship and KM had a closer relationship with TA2, BALB/c and BALB/c nu, while DBA/2 showed a distant relationship with T739, 615 and C57BL/6J, coinciding with the origins of breeds. Conclusion Each strain could be distinguished from others by using the AFLP polymorphic primers, which provides reference data for genetic quality analysis and strain determination of mice.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第2期155-159,共5页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 396 0 0 0 79
3990 0 173)
全军"九五"医药卫生科研基金资助项目 ( 96M0 83)
重庆市攻关项目
