摘要
目的 克隆大鼠肺动脉平滑肌细胞信号传导与转录活化因子亚型Ⅱ基因 (STAT2 )并对此序列进行同源性分析。方法 单纯胶原酶法培养大鼠肺动脉平滑肌细胞 ,采用RT PCR克隆大鼠肺动脉平滑肌细胞信号传导与转录活化因子亚型Ⅱ基因 (STAT2 )cDNA编码序列 ,应用PCR、四色荧光和双脱氧终止法测序 ;并对克隆序列进行同源性分析。结果 应用RT PCR克隆出大鼠肺动脉平滑肌细胞STAT2cDNA编码序列。大鼠肺动脉平滑肌细胞STAT2cDNA编码序列与Gen bank中小鼠STAT2cDNA相应序列具有高度同源性。结论 正常培养的大鼠肺动脉平滑肌细胞表达信号传导与转录活化因子亚型Ⅱ基因 (STAT2 )。
Objective To clone the gene of isoforms Ⅱof signal transducers and activators of transcription (STAT2) and analyze its homologous features Methods The sequence of STAT2 from pulmonary arterial smooth muscle cells (PASMC) was cloned with RT PCR, and sequenced with PCR, 4 color fluorescent testing and Sanger′s method The homologous features of the sequence were analyzed with the counterpart in Genbank database Results The gene of STAT2 was cloned in the PASMC, and the sequence of the gene had high homogeneity with the code region of mouse STAT2 Conclusion There exists the gene of STAT2 in normal PASMC This finding may be helpful in investigating the mechanism of signal transduction and the prevention and treatment of lung vascular diseases
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第2期104-106,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 39970 32 9)
中国博士后科学基金资助项目 ( 2 0 0 1)
第三军医大学基础部科研经费资助项目 ( 2 0 0 0 )
