摘要
目的了解医院、宾馆空调冷却塔水中军团菌污染情况。方法于2001年8月采集W市2家宾馆和4家医院的中央空调冷却塔水样,过滤空调冷却塔水的滤膜经盐酸处理后其处理液接种于GVPC平板,烛缸培养后选取可疑菌落进行生化试验、血清学试验并以PCR方法鉴定。结果W市2家医院及1家星级宾馆的中央空调冷却塔水中分离到5株军团菌,3株为嗜肺军团菌血清1型(Lp1),2株为嗜肺军团菌血清5型(Lp5)。结论应加强对医院、宾馆空调冷却塔水中军团菌的监测。
Objective To explore the contamination by Legionella pneumophila in cooling water of centralized air condi-tioning system in hospitals and hotels.Methods In Aug2001,the water from cooling towers of centralized air conditioning sys-tems in4hospitals and2hotels were sampled in W city.The membranes filtering the cooling water samples were treated by hy-drochloric acid.After treatment,the mixed treated solution was inoculated into GVPC plate and cultured in candle jar.The susepectable colonies were identified by biochemical test,serological test and PCR test respectively.Results5strains of L pneu mophila in cluding3strains of Lp1type and2strains of Lp5type were isolated from water samples of the cooling towers of centralized air conditioning systems in2hospitals and one star-grade hotel in W city.Conclusion The contamination by le-gionella in water of cooling tower of centralized air conditioning system in hospitals and hotels should be monitored closely.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2003年第1期35-36,共2页
Journal of Environment and Health
