摘要
目的 研究含氯消毒剂不能持久控制空调冷却塔水中军团菌繁殖、传播的原因 ,为寻找持久、稳固控制军团菌的方法提供依据。方法 定期跟踪调查 7个大型中央空调冷却塔水中军团菌消长情况及其有机物、细菌、藻类构成 ;模拟空调冷却塔环境条件 ,构建藻类减少和藻类增加 2种不同的军团菌生态模型 ,观察军团菌在不同微生态环境中的繁殖速率 ,同时检测空气中的军团菌。结果 空调冷却塔水中军团菌数与水中总有机碳、硝酸盐氮及藻体数(主要是绿藻门中的盘星藻、四棘藻和蓝藻门中的地中尖头藻这类体积较大的藻类 )成正比 ,含氯消毒剂不能持久杀灭军团菌。军团菌在模拟的最佳空调冷却塔水环境 (藻类增加模型 )中 ,繁殖速率可达 1h增加 1倍 ,并随藻体数增加维持指数生长状态 ;在模拟藻类减少空调冷却塔水环境中 ,加入军团菌液 1h后 ,军团菌数也可增加 1倍 ,但以后繁殖速率越来越低 ,3d后军团菌数为藻类增加生态模型的 1/ 16 0。当水中军团菌数达 4 2× 10 4/ml时 ,在空气中可检测到军团菌。结论 军团菌在 2 0℃以下的空调冷却水中不是处于休眠状态 ,而是处于失活状态。军团菌的繁殖速率和持续时间主要与空调冷却水中的藻体种类及数量密切相关 ,其次与总有机碳、硝酸盐氮有关。改变军团菌的微生态环境 ,去除冷却?
Objective To explore the cause that chlorodisinfectant unable to control proliferation and propagationg of legionella pneumophila for a long time.It is to provide guide for seeking the way that can control proliferation and propagationg of legionella pneumophila in air conditioning cooling water for a long time.Methods By periodically detecting increase or reduce of legionella pneumophila and concentration of total organic carbon (TOC), No 3 - N, total bacterial member and alga in 7 air conditioning cooling water and constituting ecopatterns of legionella pneumophila as well as tesing legionella pneumophila in air at the same time,proliferation and propagationg of legionella pneumophila were investigated in air conditioning cooling water.Results The appear or disappear and raise or down of legionella pneumophila member has mainly relation to water temperature, total organic carbon (TOC), No 3 - N, and alga member(Raphidiopsis,Pediastrum and Freubaria).In the best ecoenvironment coped from air conditioning cooling water, proliferative speed of legionella pneumophila can attain to a fold as an hour and keep the multiplicative proliferation in this experimentally 3 days. When legionella pneumophila member in the water attained to 4 2×10 4/ml, legionella pneumophila was tested in the air 30 centimetre above the water.When legionella pneumophila just was given,its proliferative speed also attained to a fold as an hour in the ecoenvironment of alga member reduce but proliferative speed was more and more down. After 3 days legionella pneumophila member in the ecoenvironment of alga member reduce only was 1/160 as that member in the ecoenvironment of alga member increase.Conclusion Legionella pneumophila in the air conditioning cooling water below 20?℃ was rather liveless than rest. The proliferative speed of legionella pneumophila was significanly related with alga member, total organic carbon (TOC) and No 3 - N besides temperature.It might be
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第5期546-549,共4页
Chinese Journal of Public Health
关键词
军团菌
微生态环境
繁殖速率
legionella pneumophila
microecoenvironment
proliferative speed