摘要
研究色谱柱的柱效降低后对高效液相色谱测定水中磺胺二甲基嘧啶色谱条件的影响。比较了新旧色谱柱的分离效果、色谱峰的对称性和峰面积,结果表明,旧色谱柱的柱效降低,对目标物的分离效果变差,目标物的吸收峰面积变小,峰形不对称。对色谱条件进行了优化试验,结果表明,对流动相水相添加0.125%的冰乙酸调节p H值为4.5后色谱峰的峰形对称性和分离效果得到改善。
When column efficiency decreased, the influence of chromatographic condition for determination of sulfamethazine in water was studied. Compared the old and new chromatographic of column separation effect, symmetry and the peak area of chromatographic peak, the results showed that the old chromatographic column effi ciency was reduced, the separation effect of the target was poorer, the target of the absorption peak area was smaller, peak shape was asymmetric. The symmetry of peak shape and seperation effect were improved as 0.125% ice acetic acid add into the mobile phase water to adjust p H value of 4.5.
出处
《化学分析计量》
CAS
2015年第3期38-40,共3页
Chemical Analysis And Meterage
基金
国家自然科学基金项目(21107100)
河南省科技厅攻关项目(142102310312)
河南省教育厅科学技术研究重点项目(13A530599)
