摘要
目的研究miR-135a对小鼠变应性鼻炎的治疗作用。方法采用卵清蛋白诱导小鼠的变应性鼻炎,生理盐水为对照。MiR-135a模拟物治疗变应性鼻炎组和模拟对照物变应性鼻炎组分别在诱导小鼠变应性鼻炎的基础上使用特异性miR-135a模拟物及模拟对照物对小鼠的变应性鼻炎进行干预。观察小鼠行为学变化并评分;酶联免疫吸附试验检测小鼠血清中卵清蛋白特异性IgE的浓度;实时聚合酶链反应检测小鼠鼻黏膜中T-bet、GATA结合蛋白3(GATA binding prolein 3,GATA-3)和干扰素γ(interferon gamma,IFN-γ)、白细胞介素4(interleukin 4,IL-4)的mRNA相对表达。结果与模拟对照物相比较,miR-135a模拟物滴鼻引起变应性鼻炎小鼠的行为学总分均低于5分,引起血清中卵清蛋白特异性IgE浓度的降低,引起鼻黏膜中GATA-3、IL-4的mRNA出现低表达,而T-bet、IFN-γ的mRNA则出现高表达。结论 MiR-135a的功能模拟治疗为microRNA用于变应性疾病的全新基因治疗提供了重要依据。
Objective To study on the effect of miR-135 a in a allergic rhinitis(AR)murine model.Methods AR was induced in mice by ovalbumin(OVA)in miR-135 a mimic AR(Mimic+OVA),mimic control AR(Mimic control+OVA)and AR(OVA)groups,while saline was used in control(Saline)group.MiR-135 a mimic AR(Mimic+OVA)and mimic control AR(Mimic control+OVA)groups were treated additionally with miR135 a mimic and mimic control respectively.Mice were graded ethologily.Serum OVA-sIgE concentration was detected with enzyme-linked immunosorbent assay(ELISA).In nasal mucosa of mice,relative mRNA expressions of T-box expressed in T cells(T-bet),GATA binding protein 3(GATA-3)and interferon gamma(IFN-γ),interleukin 4(IL-4)were detected with real-time reverse transcription-polymerase chain reaction(PCR).Results After AR intervention with miR-135 a mimic,compared with mimic control,AR mice were ethologily graded and the results were all below5,serum OVA-sIgE concentration was lower,and in nasal mucosa relative mRNA expressions of GATA-3 and IL-4 were lower,while expressions of T-bet and IFN-γwere higher.Conclusions The effect of miR-135 a in a AR murine model provided a basis for new genetic treatments of allergic diseases.
出处
《华南国防医学杂志》
CAS
2015年第7期489-492,511,共5页
Military Medical Journal of South China
基金
国家自然科学基金项目(81070766)
