摘要
目的探讨IFN-γ基因甲基化在新疆维、汉人群变应性鼻炎(Allergic rhinitis,AR)患者与正常人群中的表达。方法通过对新疆伊犁地区变应性鼻炎的流行病学调查,随机整群抽样调查符合条件的AR患者和正常人并采集外周静脉血,提取基因组DNA;亚硫酸氢盐修饰试剂盒S7820修饰DNA;甲基化特异性PCR(methylation Specific PCR,MS-PCR)法对DNA甲基化进行定性分析。结果汉族AR患者IFN-γ基因甲基化阳性率(8.5%)与正常对照组(1.7%)相比差异无统计学意义(χ2=3.229,P>0.05),维吾尔族AR患者IFN-γ基因甲基化阳性率(18.6%)与对照组(16.9%)相比差异无统计学意义(χ2=0.058,P>0.05),维吾尔族AR患者(18.6%)与汉族AR患者(8.5%)的IFN-γ基因甲基化阳性率差异无统计学意义(χ2=2.617,P>0.05),维吾尔族正常对照组(16.9%)比汉族正常对照组(1.7%)IFN-γ基因甲基化阳性率高,差异有统计学意义(χ2=8.659,P<0.05)。结论汉族正常人群IFN-γ基因低甲基化状态可能在汉族变应性鼻炎的发病过程中发挥着更为重要的作用。
To investigate the expression level of IFN-γ gene methylation in healthy peoples and allergic rhinitis（AR） patients of populations of Uighur and Han in Xinjiang,we randomly selected population via epidemiological survey in Yili area,which are consistent of the condition of healthy peoples and AR patients.In this study,the genomic DNA was extracted from peripheral blood and was modified using bisulfate modification kit S7820.Then,the methylation of DNA was analyzed by methylation specificity polymerase chain reaction（MS-PCR）.We found that the positive rate of IFN-γ gene methylation in Han were 8.5%,which was not significant differences as compared with 1.7% of healthy groups（P 0.05）.Likewise,the positive rate of methylation IFN-γ in Uighurs was 18.6%,which is not significant differences as compared with 16.9% of healthy groups（P 0.05）.Also,there are no significant differences between Uighurs（18.6%） and Han（8.5%） AR patients（P 0.05）.However,the positive rate of IFN-γ gene methylation in Uighur healthy group（16.9%） was significant higher than that of Han healthy control（1.7%）（P 0.05）.Taken these findings together,we suggest that the low methylation of IFN-γ gene in Han healthy groups may play such an important role in AR pathogenesis of Han population.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2012年第1期54-58,共5页
Immunological Journal
基金
国家自然科学基金(30960415)
新疆维吾尔自治区自然科学基金(200821136)
