摘要
目的研究IFN-α对CD56+NK细胞的细胞因子分泌、杀伤功能和细胞内信号传导的作用。方法分离健康人PBMC分别与培养液、IFN-α和IL-12培养,利用酶联免疫吸附法(ELISA)检测培养上清中IFN-γ的水平。同时采用流式细胞仪在单个细胞水平上分析IFN-α诱导IFN-γ产生的细胞亚群以及该细胞亚群对肿瘤细胞的杀伤作用和信号传导机制。结果经IFN-α刺激后,PBMC能产生低剂量的IFN-γ。细胞亚群分析的结果表明,IFN-α诱导CD56+NK细胞产生IFN-γ,但对CD4+T和CD8+T细胞无明显作用。IFN-α促进NK细胞的杀伤功能。促进STAT1和STAT4磷酸化。结论 IFN-α通过磷酸化STAT1和STAT4,增加NK细胞IFN-γ分泌和增强杀伤功能。
To elucidate the responsiveness of human NK cells to IFN-α for cytokine secretion,cytotoxicity and signal transduction,human PBMCs were isolated and co-cultured with culture medium.IFN-α or IL-12,and the levels of IFN-γ in the culture supernatants were measured by ELISA.The subsets of IFN-γ-producing lymphocytes,cytotoxicity and signal transduction were examined at single cell level by means of flow cytometry.After stimulation with IFN-α,CD56＋NK cells expressed low levels of IFN-γ.IFN-α induced cytotoxic activity as well as STAT1 and STAT4 phosphorylation.The results showed that IFN-α could induce CD56＋NK cells by phosphorylating STAT1 and STAT4 for IFN-γ secretion and cytotoxic activity. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2011年第9期752-755,759,共5页
Immunological Journal
